LOCUS       sequence-120668-        7770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax PVX_110950 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: PVX_110950-bio
FEATURES             Location/Qualifiers
     source          1..7770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3150..3205
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3204..3223)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5441..5459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5527..5631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5632..5700,5701..6492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5850..5869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6663..7251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7152..7171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7405..7422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7539..7560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7575..7580,7581..7598,7599..7605)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7613..7629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7618..7640
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7637..7653
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7637..7653
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaag aggaaaacaa ggcccctagc gcccctctgg
     1381 acgtggccca ggaaaagagc aacctgcggt acagaaacga gggcagcaag cacgtgggca
     1441 agaaaaaggc cgccagcgtg accccccagg acagcaactc taagaaaggc caggccaacg
     1501 tgaacaaggg cgagaacggc atccccgact tcgacgacag caacatggcc accgtggaag
     1561 agggccacca gagcctgcct aacgaagagg ccaaagtgaa gcccgagaca gtgaccgaca
     1621 agaaagagaa gggcgagcgc aagaagggcg gcaagaagtc tagcggcgga cagaccgtga
     1681 ccaagagcga gacagagcgg agcgatagca gcggcaccac cggcaagagg aagaagggct
     1741 ccaagaaagg cgagagccac aaaaaagaaa agaacaagca ggaaggcgcc acccccgaga
     1801 aggacgtgac catgctggtg gacggcgaac aagtgaacaa agagggcgac ttccacgtgc
     1861 ccaacgccga acagggcgtg gcccatgtgg atgtgtacgg ccctaatggc gtggacagct
     1921 gggacaccag catcagctac gagaagaacg aggccgagag cctgtaccct caagtgaccg
     1981 atcaggacgg cctgcccgtg gaaatgccta cccatggcgg agatggcacc gcccagtgga
     2041 atagccagag cgtggaagcc cctggctaca gcgacggcgt ggaacctgtg aaaggcggcg
     2101 aggccagcca cacctccgac gatagccaca atagcgaggg cgtgccaaag gccgaggaag
     2161 tggaaggcaa gaacggggcc gtgaagaatg aggccagcaa gggggagcag aaaaccccca
     2221 gcggcatcga ggaaagcaag cagaacggcg ctaaccagga tggcaacaag aaagagggct
     2281 tcagcaagaa agacctgatg aagctgttcc acgagcacat cgacctggcc agcctggtgg
     2341 aatccttcaa gagcaacgag aagctgcaga agatgagcga ggaactggtg tccttcgccg
     2401 tgaaggccta cgacggcttc ctggtgtttg tggaacaagt gaagttcttc gccaacgacg
     2461 tggtgcagat catccagaac ctgggcgcgc cgtcgacctc catcacggcc tataagagtg
     2521 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2581 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2641 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     2701 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     2761 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     2821 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     2881 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     2941 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     3001 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccgga tcactgcatc
     3061 atattctgga tgcacagaaa atggtgtgga atcatcgtta agtctagaca cgtgattaat
     3121 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     3181 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     3241 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     3301 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     3361 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     3421 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     3481 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     3541 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     3601 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     3661 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     3721 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     3781 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     3841 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     3901 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     3961 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     4021 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     4081 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     4141 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     4201 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     4261 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     4321 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     4381 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     4441 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     4501 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     4561 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     4621 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     4681 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     4741 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     4801 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4861 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4921 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4981 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5041 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     5101 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5161 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     5221 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     5281 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5341 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     5401 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     5461 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5521 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5581 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5641 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5701 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5761 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5821 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5881 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5941 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6001 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6061 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6121 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6181 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6241 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6301 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6361 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6421 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6481 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6541 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6601 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6661 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6721 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6781 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6841 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6901 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6961 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7021 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7081 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7141 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7201 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7261 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7321 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7381 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7441 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7501 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7561 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7621 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     7681 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     7741 tgctgcaggc gcagaactgg taggtatgga
//
