LOCUS       sequence-120670-        8577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax PVX_110960 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: PVX_110960-bio
FEATURES             Location/Qualifiers
     source          1..8577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3957..4012
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6248..6266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6334..6438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6439..6507,6508..7299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6657..6676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7470..8058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7959..7978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8212..8229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8346..8367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8382..8387,8388..8405,8406..8412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8420..8436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8425..8447
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8444..8460
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8444..8460
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgcca aagtgacaaa tgccgaccag ctgaaaacca
     1381 gcctgcggaa cggctttggc cagaccggaa atctggccgg acacggcgaa ggcttcagcg
     1441 tgcagggcaa cgacaacgag gaagtggact ctggcggact gggccacgcc gctaaagagg
     1501 tgctgggcga taccctggaa accggaccag ctgccgctgc cgccgcagcc aagaactgtc
     1561 ctgataatgc ccagggctgc gagggcggcg ataagtgcac acacacaaag gccacccctg
     1621 ccgtgaacca cgccacctgt aaagaggaaa agtgcgagca cagcgtggcc gactgccccg
     1681 ccaagaacag caagaacaag tgcgagacat gcaagggcaa ggactgcaac ccctgtcccg
     1741 gcgagggcga agagacagac aagaagtgtc agggccagca gtgcaagatc tgccagggca
     1801 agtgctgcgt ggaatgtaaa ggcaaggata gcaacggctg caccaagcct acctgcgaag
     1861 tgtgcggcgg aaagggcttc gtgaagtgca gcaccgaaga gatgacccac gtgtccgccg
     1921 tgtgccctct gacctgtacc accgtggatt gcgcccagtg caaagagggc ggctgcgtga
     1981 aatgcaccca gtgtggcggc gacaccagca agatggccgc ctgtaccgtg tgcaagggaa
     2041 ccggctgtac cacacaccct ggcgccacca cagtgccttg tcctgccgag tgcaccgatc
     2101 cttgcccagg cgaggattcc accccttgca ccgacccctg ctgcacacca tgtcctggcg
     2161 agacatctcc tggcggagcc gcccctggat gtacaacacc tggcccccag gaaaactgcg
     2221 ccgagctcgt gaagcccgac tgcaatccat gccctggcgt gggagatggc ggctgcaaag
     2281 gcaatagctg caccctgtgc ggaggctgct gctgtgtcgt gtgtcctggc aagggcgagg
     2341 aacagtgccc aggcaagaag tgcgaaacct gccagggaaa gggatacgtc gtgtgcaaag
     2401 gcgagaacag cgccaacgtg tgtaaagggg aaggctgcga gacttgtaaa gggaagagat
     2461 gtgtggaatg ccccggcaag gacaagaacc cctgccctgg aaaagaatgc accgtgtgcg
     2521 ccggcaaggg atgcatcaag tgccctgggg agaacaatgt ggaatgtcct ctgaagggct
     2581 gtgaagtgtg cagcgagaac ggcttcatcg agtgtaccga aaccgacaac aacaagtgcc
     2641 cccggaccaa gcccatcaag tgtcccaatg ccgtgggcgt gaactgcgcc aatgccgact
     2701 gtgatgtgtg taaacggcgg accatcaccg ccaccagcgt gacagatcag gcccctgcca
     2761 tcgagagcac cacccctgtg gtgtctcagc agacagtggc cgtgcaggct ggccctccac
     2821 ccgatcctaa tagcctgcag gaagtgaaca ccgccgagaa ggacaaggac gtgtccagca
     2881 aagaagagga agtgctggtg gaaaagggcg tggaagatga cgcccaggac ggcggccacg
     2941 atgatgatac aggcgaaggc ggcgaaaccg atagcgccaa ccaggacgac gtgtccctgg
     3001 aatacgacga cgaagaggtg gaagaagaga acgaggacgc cgagtacagc cagcagatca
     3061 ccgagacaca gaacggcaag agcaacagcc ccaccgtggg cctggaaagc ctgatctctg
     3121 atgccaccgt gcacgacgcc ctggccgaac agtgcaagga taacgtgcag ggaaagaaag
     3181 aggccgaggc cttcgtgaac accctgctgg atctgctgga cggcagcaac tctgccgccg
     3241 acaacgccat caagaacctg gccgacgata tcagccagtt tctgctgaaa ggcgcgccgt
     3301 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     3361 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     3421 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     3481 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     3541 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     3601 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     3661 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     3721 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     3781 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     3841 aagggttgaa ttccggatca ctgcatcata ttctggatgc acagaaaatg gtgtggaatc
     3901 atcgttaagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     3961 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     4021 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     4081 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     4141 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     4201 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     4261 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     4321 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     4381 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     4441 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     4501 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     4561 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     4621 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     4681 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     4741 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     4801 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     4861 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     4921 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     4981 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     5041 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     5101 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     5161 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     5221 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     5281 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     5341 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     5401 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     5461 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     5521 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     5581 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     5641 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     5701 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5761 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5821 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     5881 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     5941 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     6001 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6061 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     6121 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     6181 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     6241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6661 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6961 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     7021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8221 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8281 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8341 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8401 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8461 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     8521 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatgga
//
