LOCUS       sequence-120672-        7896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax PVX_110965 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: PVX_110965-bio
FEATURES             Location/Qualifiers
     source          1..7896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3276..3331
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3330..3349)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5567..5585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5653..5757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5758..5826,5827..6618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5976..5995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6789..7377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7278..7297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7531..7548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7665..7686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7701..7706,7707..7724,7725..7731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7739..7755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7744..7766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7763..7779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7763..7779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgata gagaagagac agtggacgcc gtgcccaacg
     1381 tgggcgtgct gaacctggaa aagcccaccc tgagagccct gctggccgaa aatcctggcg
     1441 gagtgtctga gggcgtggac aacgaggaag tggccagcga agaggtggcc aatgaggacg
     1501 tggtgtccga cgacctgccc gtggattctg tggctgctga agaggtgccc cccgaggtgg
     1561 tgcctctgga agaggtgtcc acagaggaag tgccctccga ggaagatcct tccgaagagg
     1621 acccctctga agaggatccc agcgacgatg cccctagcgc cccttctgct cctgccgccg
     1681 aagaacagaa ccaggaagcc gaagaagtgc ctccagccgc caatgccgag ccttccgatg
     1741 acagccctgt ggaagtggtg gacggcgagg acagcgtggt ggaagaagag gaaggccagc
     1801 acgtggaaca ggacgtgcca gccgtgcagg aagaagaagg acaggaagag gcccaccagg
     1861 aaagctgcag cgattgcagc gccccatgta gccctggcgc caccgagtgc gagagcaagt
     1921 gcagcgagaa ctgcaactgc gacggcgcct gccctgagga ttgtgccgtg gatgagggct
     1981 gcgaggccgt ggattgctcc gagggctccg acggatctga tggccctgcc tgtgacgcct
     2041 gccagaaaga gtctgaggac gagcagcccg aggaaggcgt ggacgctgct gctggcgtgt
     2101 cctctgaagt ggaacagcag gccgaacagg gcgtggcacc tctggctgtg gaagaggata
     2161 gcgagggcga cgagcccgag aatgaagagg actccgatgg cgaagaaacc gacgacgtgc
     2221 tgagcgagat cttccggggc ctgagaaacg tggccgacaa agaggaaacc gtgctggaag
     2281 agaacgaaga ggccgctggc gagcaggctg tggacgagaa tggacagcag caggaaggcg
     2341 aggcctacga gggcgaaggc gctggcgaag ctgctcctgg cgaagatggg gaagaagtgg
     2401 ccgagggcca gaatgccgaa gcccctagca gcgacaccag cagcaacgat gccggaatcg
     2461 gcagcgtgac caccgagaca gagaacctgg tggacgacct gatgaacctg ctgaacagcg
     2521 acaacggcgt ggaccagagc ctgaaggtgc tggcccagga tatggcccag tacttcctga
     2581 accacctgaa cgccgagagc gaggtggcag gcgcgccgtc gacctccatc acggcctata
     2641 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     2701 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     2761 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     2821 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     2881 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     2941 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     3001 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     3061 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     3121 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggatcac
     3181 tgcatcatat tctggatgca cagaaaatgg tgtggaatca tcgttaagtc tagacacgtg
     3241 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     3301 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     3361 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     3421 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     3481 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     3541 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     3601 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     3661 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     3721 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     3781 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     3841 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     3901 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     3961 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     4021 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     4081 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     4141 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     4201 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     4261 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     4321 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     4381 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     4441 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     4501 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     4561 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     4621 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     4681 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     4741 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     4801 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     4861 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     4921 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     4981 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5041 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     5101 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5161 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     5221 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     5281 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     5341 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5401 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     5461 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     5521 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     5581 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5641 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5701 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5761 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5821 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5881 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5941 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6001 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6061 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7381 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7441 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7501 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7561 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7621 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7681 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7741 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     7801 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     7861 tgccattgct gcaggcgcag aactggtagg tatgga
//
