LOCUS       sequence-120675-         478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an Neomycin-targeting gRNA and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Chiou SH, Winters IP, Wang J, Naranjo S, Dudgeon C, Tamburini FB, 
            Brady JJ, Yang D, Gruner BM, Chuang CH, Caswell DR, Zeng H, Chu P, 
            Kim GE, Carpizo DR, Kim SK, Winslow MM
  TITLE     Pancreatic cancer modeling using retrograde viral vector delivery 
            and in vivo CRISPR/Cas9-mediated somatic genome editing.
  JOURNAL   Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. 
            Epub 2015 Jul 15.
  PUBMED    26178787
REFERENCE   2  (bases 1 to 478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.264861.115"; journalName: "Genes Dev"; date: 
            "2015-07-15- 15"; volume: "29"; issue: "14"; pages: "1576-85"
COMMENT     Sequence Label: Lenti-sgNeo/Cre
FEATURES             Location/Qualifiers
     source          1..478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(51..126)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(148..396)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(206..225)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(376..396)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tagaatgcgg ccgccatgtt aattaagcca taggcgcgcc aaacaaaaaa gcaccgactc
       61 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
      121 taaaacccgc attgcatcag ccatgacggt gtttggtcct ttccacaaga tatataaagc
      181 caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa
      241 ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa
      301 tatctttgtg tttacagtca aattaattcc aattatctct ctaacagcct tgtatcgtat
      361 atgcaaatat gaaggaatca tgggaaatag gccctcacag tttaaacagt tcctgcaggg
      421 ctagagcggg cccggtacta accaaactgg atctctgctg tccctgtaat aaacccga
//
