LOCUS       sequence-120676-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an Neomycin-targeting gRNA and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Chiou SH, Winters IP, Wang J, Naranjo S, Dudgeon C, Tamburini FB, 
            Brady JJ, Yang D, Gruner BM, Chuang CH, Caswell DR, Zeng H, Chu P, 
            Kim GE, Carpizo DR, Kim SK, Winslow MM
  TITLE     Pancreatic cancer modeling using retrograde viral vector delivery 
            and in vivo CRISPR/Cas9-mediated somatic genome editing.
  JOURNAL   Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. 
            Epub 2015 Jul 15.
  PUBMED    26178787
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.264861.115"; journalName: "Genes Dev"; date: 
            "2015-07-15- 15"; volume: "29"; issue: "14"; pages: "1576-85"
COMMENT     Sequence Label: Lenti-sgNeo/Cre
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..273
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     25..45
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     196..215
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        295..370
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(458..481)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccctgcagga actgtttaaa ctgtgagggc ctatttccca tgattccttc atatttgcat
       61 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
      121 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      181 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      241 gctttatata tcttgtggaa aggaccaaac accgtcatgg ctgatgcaat gcgggtttta
      301 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      361 gagtcggtgc ttttttgttt ggcgcgccta tggcttaatt aacatggcgg ccgcattcta
      421 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
      481 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      541 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      601 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      661 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
      721 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
      781 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggg
//
