LOCUS       sequence-120678-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SREBF2
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgctc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg gggaggcggc aatggcagtg ccaccaccca gcttaacaat catctgctgg
      241 cagtcgttgc aggagcgccg gtcgcccacc ttctccaggg tgcgggcagc ttcggccagc
      301 agcactgccc gctggcccgg ggaggagagg aaggagaggg gcaggtggcg gcaggccagc
      361 aggatggcgg tggcccgctc tcgctggccg ggccaggcat ccacctctcc gtgcttggtg
      421 ctctgcgtgg tgcgccgccg caggctgtgt tccagcagct ggtgggtgcg ggtggggctg
      481 gctcctgcca tcaggcgcac ggtggcttca tgcaggaaca ccttgcggta tgctgggcgg
      541 aagctgtgtg ccagcctgcg caggctgccc aggtcccgtt ggaagcccgc cagttcagcg
      601 cctgacgcgt ggtaggtctc ccccacagcc tggctggcac tggcctgttt ttgccagagc
      661 gctgtccgta gcgacagtag caggtcacag gtgagcagct ggaccacgtg gttgagggca
      721 gggtcagagg tggccccact gacgttgagg ctgctccata ggtggccact ggccctctcg
      781 caatggcaga aggaactctg ctgcccatct gctttcccag ggagtgaggc atgcatggct
      841 ctgcaggcat ggaagatggc cttcaccagg gggctctctg tcacttccag ggccttgggg
      901 atgcgttcca ctttggtaaa atgagagcgc acagctgcat cgtctccctg gagccagctg
      961 atggccacag tg
//
