LOCUS       sequence-120682-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PDGFRB
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtacaggaag ctatcctctg cttccgcccg aggcgcaggg caccccgaat
      241 ccggcaactg ttccagctct ggctccggct ccacctggag ctcaagctgg ggctctggct
      301 ctggttcgtc ctggggctcc agggggctgt cacaggagat ggttgaggag gtgttgactt
      361 cattcagggt ggagctggct aggctggggg aaccctccag tgggccctcg tcagcaacct
      421 cgggtttggg gtcaggcagg gggatgatat agtcgttgtc accctcattg ggctgcacgg
      481 cagtatagag gacggagctg gtgtccaggg gagatcggag gccatggaac ccaggcaagc
      541 gggcctggga ccgaaggatg gctgggtggt cactcctcag aaactcctca tccacctgct
      601 ggtacttctt tttgtaacct tcgcccaaca gtctctcgag aagcagcacc agctgggaga
      661 aggggggccg aatctcaaac ttctcttccc agcacttctg catgatctca tagatctcgt
      721 cggaggcatg ggcaggctgg gccatgcggt aaccccgttt gatggcattg tagaactgct
      781 cgttcatggg cagctctggg taaggggtgc cacccaaggt gaagatctcc cagagcagga
      841 tcccgaagga ccacacgtcg ctcagggtgg tgtagaggct gttgaagatg ctctccggag
      901 ccatccactt taaaggcaaa aaggtgctgc ctttggagat gtaattcgag tcccgcatga
      961 tgtctcgagc caggccaaag tc
//
