LOCUS       sequence-120685-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 NFX1
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagctgg gtccaagtcc tggacgtcaa aatagtcaat tattggctcc ttggttattt
      241 tctgtaaatt actgctccca ggattcttgt ctgactgatg tctgtgatga ggaatcggtg
      301 gtggaggccg tgcctgcatt tccctttcaa gcacacctgt cagcgtggta ggaggacaaa
      361 cggacttccc cctgatggca gtgaccacca cattgcgctt cggttcactg tcatagctca
      421 cgctctccag gccataaact tgggccaagt catggatgat ccggcggtgg tctctgttca
      481 tgggagggaa gctgtggctt ttcttactat tctttccctt attcacggcc tccacgaggg
      541 tttccatttc cttctcaacg tcactgacaa actttaagtc cttcctggca tcttctttca
      601 aactatcact gaattttgac cctgaagaac gtatattgaa aggatcagaa tcctcactga
      661 tatgaaatgc ctctgctaat ctctttttcc tttccaaggc tgaacactcc tcatcacact
      721 ccagcctggc ttgatgaact tcctttttgg taattaactt gctgatctcc actgaacctc
      781 caagctgcat gtctgttatc ttagaggcca tggagattgc agctattctt tgataagtac
      841 tagatgcttc agagcaaatc accatctctt ttcttcgtcc acattcacac tgtagctcta
      901 c
//
