LOCUS       sequence-120704-        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synchronize trafficking of Ecadherin from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_SBP-EGFP-Ecadherin
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1401..1427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          1946..2175
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2158..2177
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(2233..2807,2808..2810,2811..2819)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2400..2417)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2627..2646
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     sig_peptide     2833..2892
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             2893..3003
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(3013..3015,3016..3018,3019..3729)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3013..3015,3016..3018,3019..3729)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3058..3079)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3319..3338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3666..3687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6067..6188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6104..6123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6158..6177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6195..6650)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6332..6353)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6544..6563
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6677..6781
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6783..7140
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6783..6974
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6806..6826)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6991..7126
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7175..7969
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7175..7969
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7229..7248)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7839..7858
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8157..8176)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8201..8248
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8577..9165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9066..9085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgtac gtctccggat tcgaattaat tcggatccgt gatgcacagg aggagagcca
      661 gggcctgtcg ggaagatcaa aagccagtca tcgatgatca gcgcgacctt atctccaaca
      721 atgagcaact gcccatgctg ggccggcgcc ctggggcccc ggagagcaag tgcagccgcg
      781 gagccctgta cacaggcttt tccatcctgg tgactctgct cctcgctggc caggccacca
      841 ccgcctactt cctgtaccag cagcagggcc ggctggacaa actgacagtc acctcccaga
      901 acctgcagct cgagatggac cctagcaaag actcaaaagc tcaggtgtcc gctgccgagg
      961 ctggcattac tggaacatgg tacaatcagc tcgggagcac ctttattgtg actgctggag
     1021 ccgatggagc cctcaccgga acatacgaat ctgctgtggg aaacgccgaa tcacggtacg
     1081 tcctcactgg ccgatacgat agtgcccctg ccaccgacgg atctgggact gccctgggat
     1141 ggactgtcgc ttggaaaaac aactaccgga atgctcattc tgccacaaca tggagtggac
     1201 agtacgtggg aggcgctgag gctagaatca atacacagtg gctgctcaca tctggcacaa
     1261 ccgaggcaaa tgcttggaaa tccaccctgg tgggacatga cacattcacc aaagtgaaac
     1321 cctccgccgc ttcaatcgat gccgccaaaa aagccggagt caacaacggc aatcctctgg
     1381 atgccgtcca gcaggtcgac tatccgtacg acgtaccaga ctacgcactc gacctgcagc
     1441 tggagaacct gcgcatgaag cttcccaagc ctcccaagcc tgtgagcaag atgcgcatgg
     1501 ccaccccgct gctgatgcat gcgctgccca tgggagccct gccccagggg cccatgcaga
     1561 atgccaccaa gtatggcaac atgacagagg accatgtgat gcacctgctc cagaatgctg
     1621 accccctgaa ggtgtacccg ccactgaagg ggagcttccc ggagaacctg agacacctta
     1681 agaacaccat ggagaccata gactggaagg tctttgagag ctggatgcac cattggctcc
     1741 tgtttgaaat gagcaggcac tccttggagc aaaagcccac tgacgctcca ccgaaagagt
     1801 cactggaact ggaggacccg tcttctgggc tgggtgtgac caagcaggat ctgggcccag
     1861 tccccatgtg aggatccgcg gccgcataga taactgatcc agtgtgctgg aattaattcg
     1921 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct
     1981 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
     2041 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
     2101 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
     2161 cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg
     2221 cggccaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     2281 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2341 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     2401 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     2461 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     2521 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     2581 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     2641 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     2701 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     2761 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc
     2821 gggaggcgcg ccatgtacag gatgcaactc ctgtcttgca ttgcactaag tcttgcactt
     2881 gtcacgaatt ccgacgagaa gaccactggt tggcgaggtg gacacgttgt tgaaggactg
     2941 gctggggaac ttgaacaact tcgtgcacga ctggagcatc acccacaagg tcaacgtgaa
     3001 ccacctgcag gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3061 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3181 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3481 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     3541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3601 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     3661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3721 ctgtacaagg gccggccaga ctgggtcatc cctcccatca gctgccccga aaatgaaaag
     3781 ggtgaattcc caaagaacct ggttcagatc aaatccaaca gggacaaaga aacaaaggtt
     3841 ttctacagca tcaccggcca aggagctgac aaaccccccg ttggcgtttt catcattgag
     3901 agggagacag gctggctgaa agtgacacag cctctggata gagaagccat tgccaagtac
     3961 atcctctatt ctcatgccgt gtcatcaaat ggggaagcgg tggaggatcc catggagata
     4021 gtgatcacag tgacagatca gaatgacaac aggccagagt ttacccagga ggtgtttgag
     4081 ggatccgttg cagaaggcgc tgttccagga acctccgtga tgaaggtctc agccaccgat
     4141 gcagacgatg acgtcaacac ctacaacgct gccatcgcct acaccatcgt cagccaggat
     4201 cctgagctgc ctcacaaaaa catgttcact gtcaataggg acaccggggt catcagtgtg
     4261 ctcacctctg ggctggaccg agagagttac cctacataca ctctggtggt tcaggctgct
     4321 gaccttcaag gcgaaggctt gagcacaaca gccaaggctg tgatcactgt caaggatatt
     4381 aatgacaacg ctcctgtctt caacccgagc acgtatcagg gtcaagtgcc tgagaatgag
     4441 gtcaatgccc ggatcgccac actcaaagtg accgatgatg atgcccccaa cactccggcg
     4501 tggaaagctg tgtacaccgt agtcaacgat cctgaccagc agttcgttgt tgtcacagac
     4561 cccacgacca atgatggcat tttgaaaaca gccaagggct tggattttga ggccaagcag
     4621 caatacatcc ttcatgtgag agtggagaac gaggaaccct ttgaggggtc tcttgtccct
     4681 tccacagcca ctgtcactgt ggacgtggta gacgtgaatg aagcccccat ctttatgcct
     4741 gcggagagga gagtcgaagt gcccgaagac tttggtgtgg gtcaggaaat cacatcttat
     4801 accgctcgag agccggacac gttcatggat cagaagatca cgtatcggat ttggagggac
     4861 actgccaact ggctggagat taacccagag actggtgcca ttttcacgcg cgctgagatg
     4921 gacagagaag acgctgagca tgtgaagaac agcacatatg tagctctcat catcgccaca
     4981 gatgatggtt cacccattgc cactggcacg ggcactcttc tcctggtcct gttagacgtc
     5041 aatgacaacg ctcccatccc agaacctcga aacatgcagt tctgccagag gaacccacag
     5101 cctcatatca tcaccatctt ggatccagac cttcccccca acacgtcccc ctttactgct
     5161 gagctaaccc atggggccag cgtcaactgg accattgagt ataatgacgc agctcaagaa
     5221 tctctcattt tgcaaccaag aaaggactta gagattggcg aatacaaaat ccatctcaag
     5281 ctcgcggata accagaacaa agaccaggtg accacgttgg acgtccatgt gtgtgactgt
     5341 gaagggacgg tcaacaactg catgaaggcg ggaatcgtgg cagcaggatt gcaagttcct
     5401 gccatcctcg gaatccttgg agggatcctc gccctgctga ttctgatcct gctgctccta
     5461 ctgtttctac ggaggagaac ggtggtcaaa gagcccctgc tgccaccaga tgatgatacc
     5521 cgggacaatg tgtattacta tgatgaagaa ggaggtggag aagaagacca ggactttgat
     5581 ttgagccagc tgcacagggg cctggatgcc cgaccggaag tgactcgaaa tgatgtggct
     5641 cccaccctca tgagcgtgcc ccagtatcgt ccccgtcctg ccaatcctga tgaaattgga
     5701 aacttcatcg atgaaaacct gaaggcagcc gacagcgacc ccacggcacc cccttacgac
     5761 tctctgttgg tgttcgatta cgagggcagt ggttctgaag ccgctagcct gagctcactg
     5821 aactcctctg agtcggatca ggaccaggac tacgattatc tgaacgagtg gggcaaccga
     5881 ttcaagaagc tggcggacat gtacggcggt ggcgaagacg actagggcct cgagggccaa
     5941 ttaattaact ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     6001 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6061 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6181 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     6241 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     6301 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     6361 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     6421 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     6481 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6541 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6601 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     6661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6781 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6841 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6901 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6961 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7021 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7081 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7141 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     7201 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     7261 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     7321 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     7381 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7441 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7501 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7561 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7621 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7681 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7741 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7801 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7861 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7921 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7981 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8041 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8101 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     8161 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     8221 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     8281 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     8341 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     8401 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     8461 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8521 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8581 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8641 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8701 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8761 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8821 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8881 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8941 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9001 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9061 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9241 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
