LOCUS       sequence-120705-        9270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synchronize trafficking of Ecadherin from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_SBP-mCherry-Ecadherin
FEATURES             Location/Qualifiers
     source          1..9270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1401..1427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          1946..2175
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2158..2177
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(2233..2807,2808..2810,2811..2819)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2400..2417)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2627..2646
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     sig_peptide     2833..2892
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             2893..3003
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             3013..3720
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3158..3176)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3426..3445
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6058..6179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6095..6114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6149..6168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6186..6641)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6323..6344)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6535..6554
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6668..6772
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6774..7131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6774..6965
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6797..6817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6982..7117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7166..7960
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7166..7960
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7220..7239)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7830..7849
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8148..8167)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8192..8239
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8568..9156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9057..9076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgtac gtctccggat tcgaattaat tcggatccgt gatgcacagg aggagagcca
      661 gggcctgtcg ggaagatcaa aagccagtca tcgatgatca gcgcgacctt atctccaaca
      721 atgagcaact gcccatgctg ggccggcgcc ctggggcccc ggagagcaag tgcagccgcg
      781 gagccctgta cacaggcttt tccatcctgg tgactctgct cctcgctggc caggccacca
      841 ccgcctactt cctgtaccag cagcagggcc ggctggacaa actgacagtc acctcccaga
      901 acctgcagct cgagatggac cctagcaaag actcaaaagc tcaggtgtcc gctgccgagg
      961 ctggcattac tggaacatgg tacaatcagc tcgggagcac ctttattgtg actgctggag
     1021 ccgatggagc cctcaccgga acatacgaat ctgctgtggg aaacgccgaa tcacggtacg
     1081 tcctcactgg ccgatacgat agtgcccctg ccaccgacgg atctgggact gccctgggat
     1141 ggactgtcgc ttggaaaaac aactaccgga atgctcattc tgccacaaca tggagtggac
     1201 agtacgtggg aggcgctgag gctagaatca atacacagtg gctgctcaca tctggcacaa
     1261 ccgaggcaaa tgcttggaaa tccaccctgg tgggacatga cacattcacc aaagtgaaac
     1321 cctccgccgc ttcaatcgat gccgccaaaa aagccggagt caacaacggc aatcctctgg
     1381 atgccgtcca gcaggtcgac tatccgtacg acgtaccaga ctacgcactc gacctgcagc
     1441 tggagaacct gcgcatgaag cttcccaagc ctcccaagcc tgtgagcaag atgcgcatgg
     1501 ccaccccgct gctgatgcat gcgctgccca tgggagccct gccccagggg cccatgcaga
     1561 atgccaccaa gtatggcaac atgacagagg accatgtgat gcacctgctc cagaatgctg
     1621 accccctgaa ggtgtacccg ccactgaagg ggagcttccc ggagaacctg agacacctta
     1681 agaacaccat ggagaccata gactggaagg tctttgagag ctggatgcac cattggctcc
     1741 tgtttgaaat gagcaggcac tccttggagc aaaagcccac tgacgctcca ccgaaagagt
     1801 cactggaact ggaggacccg tcttctgggc tgggtgtgac caagcaggat ctgggcccag
     1861 tccccatgtg aggatccgcg gccgcataga taactgatcc agtgtgctgg aattaattcg
     1921 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct
     1981 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
     2041 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
     2101 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
     2161 cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg
     2221 cggccaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     2281 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2341 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     2401 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     2461 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     2521 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     2581 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     2641 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     2701 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     2761 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc
     2821 gggaggcgcg ccatgtacag gatgcaactc ctgtcttgca ttgcactaag tcttgcactt
     2881 gtcacgaatt ccgacgagaa gaccactggt tggcgaggtg gacacgttgt tgaaggactg
     2941 gctggggaac ttgaacaact tcgtgcacga ctggagcatc acccacaagg tcaacgtgaa
     3001 ccacctgcag gtatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3061 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3121 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3181 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3241 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3301 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3361 tccctacagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3421 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3481 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3541 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3601 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3661 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3721 ggccggccag actgggtcat ccctcccatc agctgccccg aaaatgaaaa gggtgaattc
     3781 ccaaagaacc tggttcagat caaatccaac agggacaaag aaacaaaggt tttctacagc
     3841 atcaccggcc aaggagctga caaacccccc gttggcgttt tcatcattga gagggagaca
     3901 ggctggctga aagtgacaca gcctctggat agagaagcca ttgccaagta catcctctat
     3961 tctcatgccg tgtcatcaaa tggggaagcg gtggaggatc ccatggagat agtgatcaca
     4021 gtgacagatc agaatgacaa caggccagag tttacccagg aggtgtttga gggatccgtt
     4081 gcagaaggcg ctgttccagg aacctccgtg atgaaggtct cagccaccga tgcagacgat
     4141 gacgtcaaca cctacaacgc tgccatcgcc tacaccatcg tcagccagga tcctgagctg
     4201 cctcacaaaa acatgttcac tgtcaatagg gacaccgggg tcatcagtgt gctcacctct
     4261 gggctggacc gagagagtta ccctacatac actctggtgg ttcaggctgc tgaccttcaa
     4321 ggcgaaggct tgagcacaac agccaaggct gtgatcactg tcaaggatat taatgacaac
     4381 gctcctgtct tcaacccgag cacgtatcag ggtcaagtgc ctgagaatga ggtcaatgcc
     4441 cggatcgcca cactcaaagt gaccgatgat gatgccccca acactccggc gtggaaagct
     4501 gtgtacaccg tagtcaacga tcctgaccag cagttcgttg ttgtcacaga ccccacgacc
     4561 aatgatggca ttttgaaaac agccaagggc ttggattttg aggccaagca gcaatacatc
     4621 cttcatgtga gagtggagaa cgaggaaccc tttgaggggt ctcttgtccc ttccacagcc
     4681 actgtcactg tggacgtggt agacgtgaat gaagccccca tctttatgcc tgcggagagg
     4741 agagtcgaag tgcccgaaga ctttggtgtg ggtcaggaaa tcacatctta taccgctcga
     4801 gagccggaca cgttcatgga tcagaagatc acgtatcgga tttggaggga cactgccaac
     4861 tggctggaga ttaacccaga gactggtgcc attttcacgc gcgctgagat ggacagagaa
     4921 gacgctgagc atgtgaagaa cagcacatat gtagctctca tcatcgccac agatgatggt
     4981 tcacccattg ccactggcac gggcactctt ctcctggtcc tgttagacgt caatgacaac
     5041 gctcccatcc cagaacctcg aaacatgcag ttctgccaga ggaacccaca gcctcatatc
     5101 atcaccatct tggatccaga ccttcccccc aacacgtccc cctttactgc tgagctaacc
     5161 catggggcca gcgtcaactg gaccattgag tataatgacg cagctcaaga atctctcatt
     5221 ttgcaaccaa gaaaggactt agagattggc gaatacaaaa tccatctcaa gctcgcggat
     5281 aaccagaaca aagaccaggt gaccacgttg gacgtccatg tgtgtgactg tgaagggacg
     5341 gtcaacaact gcatgaaggc gggaatcgtg gcagcaggat tgcaagttcc tgccatcctc
     5401 ggaatccttg gagggatcct cgccctgctg attctgatcc tgctgctcct actgtttcta
     5461 cggaggagaa cggtggtcaa agagcccctg ctgccaccag atgatgatac ccgggacaat
     5521 gtgtattact atgatgaaga aggaggtgga gaagaagacc aggactttga tttgagccag
     5581 ctgcacaggg gcctggatgc ccgaccggaa gtgactcgaa atgatgtggc tcccaccctc
     5641 atgagcgtgc cccagtatcg tccccgtcct gccaatcctg atgaaattgg aaacttcatc
     5701 gatgaaaacc tgaaggcagc cgacagcgac cccacggcac ccccttacga ctctctgttg
     5761 gtgttcgatt acgagggcag tggttctgaa gccgctagcc tgagctcact gaactcctct
     5821 gagtcggatc aggaccagga ctacgattat ctgaacgagt ggggcaaccg attcaagaag
     5881 ctggcggaca tgtacggcgg tggcgaagac gactagggcc tcgagggcca attaattaac
     5941 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     6001 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     6061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     6181 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6241 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6301 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6361 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6421 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6481 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6541 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6601 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     6661 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6721 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     6781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     7141 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     7201 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     7261 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     7321 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     7381 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     7441 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     7501 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     7561 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     7621 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     7681 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     7741 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     7801 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     7861 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7921 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7981 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     8041 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8101 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     8161 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     8221 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     8281 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     8341 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     8401 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8701 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9241 tctgtggata accgtattac cgccatgcat
//
