LOCUS       sequence-120706-       10492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ecadherin reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: pCDH_SBP-EGFP-Ecadherin
FEATURES             Location/Qualifiers
     source          1..10492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2005..2208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2159..2179
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2205..2229
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     sig_peptide     2305..2364
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             2365..2475
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(2485..2487,2488..2490,2491..3201)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2485..2487,2488..2490,2491..3201)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2530..2551)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2791..2810)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3138..3159
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5462..5673
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5686..5954
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5981..6580
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5981..6000)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6477..6497
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6587..7175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6640..6660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7058..7069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7249..7482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7554..7675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7591..7610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7645..7664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7694..7829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7867..7883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7867..7883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7880..7902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7891..7907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7915..7921,7922..7939,7940..7945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7960..7981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8098..8115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8269..8857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9028..9819,9820..9888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9651..9670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9889..9993)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10061..10079
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10117..10139)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10239..10258
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10452..10474
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10466..10483
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10467..10483
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcaccggt ggatccggcg cgccatgtac aggatgcaac tcctgtcttg cattgcacta
     2341 agtcttgcac ttgtcacgaa ttccgacgag aagaccactg gttggcgagg tggacacgtt
     2401 gttgaaggac tggctgggga acttgaacaa cttcgtgcac gactggagca tcacccacaa
     2461 ggtcaacgtg aaccacctgc aggtatggtg agcaagggcg aggagctgtt caccggggtg
     2521 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2581 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2641 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     2701 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2761 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2821 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2881 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2941 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3001 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3061 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3121 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3181 ggcatggacg agctgtacaa gggccggcca gactgggtca tccctcccat cagctgcccc
     3241 gaaaatgaaa agggtgaatt cccaaagaac ctggttcaga tcaaatccaa cagggacaaa
     3301 gaaacaaagg ttttctacag catcaccggc caaggagctg acaaaccccc cgttggcgtt
     3361 ttcatcattg agagggagac aggctggctg aaagtgacac agcctctgga tagagaagcc
     3421 attgccaagt acatcctcta ttctcatgcc gtgtcatcaa atggggaagc ggtggaggat
     3481 cccatggaga tagtgatcac agtgacagat cagaatgaca acaggccaga gtttacccag
     3541 gaggtgtttg agggatccgt tgcagaaggc gctgttccag gaacctccgt gatgaaggtc
     3601 tcagccaccg atgcagacga tgacgtcaac acctacaacg ctgccatcgc ctacaccatc
     3661 gtcagccagg atcctgagct gcctcacaaa aacatgttca ctgtcaatag ggacaccggg
     3721 gtcatcagtg tgctcacctc tgggctggac cgagagagtt accctacata cactctggtg
     3781 gttcaggctg ctgaccttca aggcgaaggc ttgagcacaa cagccaaggc tgtgatcact
     3841 gtcaaggata ttaatgacaa cgctcctgtc ttcaacccga gcacgtatca gggtcaagtg
     3901 cctgagaatg aggtcaatgc ccggatcgcc acactcaaag tgaccgatga tgatgccccc
     3961 aacactccgg cgtggaaagc tgtgtacacc gtagtcaacg atcctgacca gcagttcgtt
     4021 gttgtcacag accccacgac caatgatggc attttgaaaa cagccaaggg cttggatttt
     4081 gaggccaagc agcaatacat ccttcatgtg agagtggaga acgaggaacc ctttgagggg
     4141 tctcttgtcc cttccacagc cactgtcact gtggacgtgg tagacgtgaa tgaagccccc
     4201 atctttatgc ctgcggagag gagagtcgaa gtgcccgaag actttggtgt gggtcaggaa
     4261 atcacatctt ataccgctcg agagccggac acgttcatgg atcagaagat cacgtatcgg
     4321 atttggaggg acactgccaa ctggctggag attaacccag agactggtgc cattttcacg
     4381 cgcgctgaga tggacagaga agacgctgag catgtgaaga acagcacata tgtagctctc
     4441 atcatcgcca cagatgatgg ttcacccatt gccactggca cgggcactct tctcctggtc
     4501 ctgttagacg tcaatgacaa cgctcccatc ccagaacctc gaaacatgca gttctgccag
     4561 aggaacccac agcctcatat catcaccatc ttggatccag accttccccc caacacgtcc
     4621 ccctttactg ctgagctaac ccatggggcc agcgtcaact ggaccattga gtataatgac
     4681 gcagctcaag aatctctcat tttgcaacca agaaaggact tagagattgg cgaatacaaa
     4741 atccatctca agctcgcgga taaccagaac aaagaccagg tgaccacgtt ggacgtccat
     4801 gtgtgtgact gtgaagggac ggtcaacaac tgcatgaagg cgggaatcgt ggcagcagga
     4861 ttgcaagttc ctgccatcct cggaatcctt ggagggatcc tcgccctgct gattctgatc
     4921 ctgctgctcc tactgtttct acggaggaga acggtggtca aagagcccct gctgccacca
     4981 gatgatgata cccgggacaa tgtgtattac tatgatgaag aaggaggtgg agaagaagac
     5041 caggactttg atttgagcca gctgcacagg ggcctggatg cccgaccgga agtgactcga
     5101 aatgatgtgg ctcccaccct catgagcgtg ccccagtatc gtccccgtcc tgccaatcct
     5161 gatgaaattg gaaacttcat cgatgaaaac ctgaaggcag ccgacagcga ccccacggca
     5221 cccccttacg actctctgtt ggtgttcgat tacgagggca gtggttctga agccgctagc
     5281 ctgagctcac tgaactcctc tgagtcggat caggaccagg actacgatta tctgaacgag
     5341 tggggcaacc gattcaagaa gctggcggac atgtacggcg gtggcgaaga cgactagggc
     5401 ctcgagggcc aattaattaa gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5461 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5521 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5581 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5641 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5701 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5761 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5821 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5881 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5941 cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac
     6001 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6061 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6121 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6181 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6241 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6301 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6361 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6421 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6481 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6541 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga
     6601 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6661 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6721 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6781 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6841 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6901 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6961 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7021 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7081 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7141 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     7201 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7261 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7501 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7681 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7801 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     7861 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7921 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7981 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8041 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8101 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8161 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8221 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8341 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8401 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8461 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8521 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8581 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8641 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8701 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8761 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10021 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10081 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10141 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10201 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10261 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10321 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10381 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10441 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
