LOCUS       sequence-120707-       10480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ecadherin reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: pCDH_SBP-mCherry-Ecadherin
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2005..2208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2159..2179
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2205..2229
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     sig_peptide     2305..2364
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             2365..2475
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             2485..3189
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2627..2645)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2895..2914
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5450..5661
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5674..5942
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5969..6568
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5969..5988)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6465..6485
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6575..7163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6628..6648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7046..7057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7237..7470
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7542..7663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7579..7598)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7633..7652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7682..7817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7855..7871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7855..7871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7868..7890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7879..7895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7903..7909,7910..7927,7928..7933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7948..7969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8086..8103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8257..8845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9016..9807,9808..9876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9639..9658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9877..9981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10049..10067
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10105..10127)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10227..10246
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10440..10462
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10454..10471
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10455..10471
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcaccggt ggatccggcg cgccatgtac aggatgcaac tcctgtcttg cattgcacta
     2341 agtcttgcac ttgtcacgaa ttccgacgag aagaccactg gttggcgagg tggacacgtt
     2401 gttgaaggac tggctgggga acttgaacaa cttcgtgcac gactggagca tcacccacaa
     2461 ggtcaacgtg aaccacctgc aggtgtgagc aagggcgagg aggataacat ggccatcatc
     2521 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2581 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2641 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2701 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2761 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2821 caggactcct ccctacagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2881 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     2941 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3001 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3061 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     3121 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3181 ctgtacaagg gccggccaga ctgggtcatc cctcccatca gctgccccga aaatgaaaag
     3241 ggtgaattcc caaagaacct ggttcagatc aaatccaaca gggacaaaga aacaaaggtt
     3301 ttctacagca tcaccggcca aggagctgac aaaccccccg ttggcgtttt catcattgag
     3361 agggagacag gctggctgaa agtgacacag cctctggata gagaagccat tgccaagtac
     3421 atcctctatt ctcatgccgt gtcatcaaat ggggaagcgg tggaggatcc catggagata
     3481 gtgatcacag tgacagatca gaatgacaac aggccagagt ttacccagga ggtgtttgag
     3541 ggatccgttg cagaaggcgc tgttccagga acctccgtga tgaaggtctc agccaccgat
     3601 gcagacgatg acgtcaacac ctacaacgct gccatcgcct acaccatcgt cagccaggat
     3661 cctgagctgc ctcacaaaaa catgttcact gtcaataggg acaccggggt catcagtgtg
     3721 ctcacctctg ggctggaccg agagagttac cctacataca ctctggtggt tcaggctgct
     3781 gaccttcaag gcgaaggctt gagcacaaca gccaaggctg tgatcactgt caaggatatt
     3841 aatgacaacg ctcctgtctt caacccgagc acgtatcagg gtcaagtgcc tgagaatgag
     3901 gtcaatgccc ggatcgccac actcaaagtg accgatgatg atgcccccaa cactccggcg
     3961 tggaaagctg tgtacaccgt agtcaacgat cctgaccagc agttcgttgt tgtcacagac
     4021 cccacgacca atgatggcat tttgaaaaca gccaagggct tggattttga ggccaagcag
     4081 caatacatcc ttcatgtgag agtggagaac gaggaaccct ttgaggggtc tcttgtccct
     4141 tccacagcca ctgtcactgt ggacgtggta gacgtgaatg aagcccccat ctttatgcct
     4201 gcggagagga gagtcgaagt gcccgaagac tttggtgtgg gtcaggaaat cacatcttat
     4261 accgctcgag agccggacac gttcatggat cagaagatca cgtatcggat ttggagggac
     4321 actgccaact ggctggagat taacccagag actggtgcca ttttcacgcg cgctgagatg
     4381 gacagagaag acgctgagca tgtgaagaac agcacatatg tagctctcat catcgccaca
     4441 gatgatggtt cacccattgc cactggcacg ggcactcttc tcctggtcct gttagacgtc
     4501 aatgacaacg ctcccatccc agaacctcga aacatgcagt tctgccagag gaacccacag
     4561 cctcatatca tcaccatctt ggatccagac cttcccccca acacgtcccc ctttactgct
     4621 gagctaaccc atggggccag cgtcaactgg accattgagt ataatgacgc agctcaagaa
     4681 tctctcattt tgcaaccaag aaaggactta gagattggcg aatacaaaat ccatctcaag
     4741 ctcgcggata accagaacaa agaccaggtg accacgttgg acgtccatgt gtgtgactgt
     4801 gaagggacgg tcaacaactg catgaaggcg ggaatcgtgg cagcaggatt gcaagttcct
     4861 gccatcctcg gaatccttgg agggatcctc gccctgctga ttctgatcct gctgctccta
     4921 ctgtttctac ggaggagaac ggtggtcaaa gagcccctgc tgccaccaga tgatgatacc
     4981 cgggacaatg tgtattacta tgatgaagaa ggaggtggag aagaagacca ggactttgat
     5041 ttgagccagc tgcacagggg cctggatgcc cgaccggaag tgactcgaaa tgatgtggct
     5101 cccaccctca tgagcgtgcc ccagtatcgt ccccgtcctg ccaatcctga tgaaattgga
     5161 aacttcatcg atgaaaacct gaaggcagcc gacagcgacc ccacggcacc cccttacgac
     5221 tctctgttgg tgttcgatta cgagggcagt ggttctgaag ccgctagcct gagctcactg
     5281 aactcctctg agtcggatca ggaccaggac tacgattatc tgaacgagtg gggcaaccga
     5341 ttcaagaagc tggcggacat gtacggcggt ggcgaagacg actagggcct cgagggccaa
     5401 ttaattaagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5461 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5521 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5581 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5641 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5701 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5761 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5821 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5881 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5941 tccaagctgt gaccggcgcc tacgctagat gaccgagtac aagcccacgg tgcgcctcgc
     6001 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6061 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6121 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6181 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6241 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6301 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6361 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6421 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6481 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6541 gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg
     6601 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6661 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6721 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6781 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6841 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6901 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6961 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7021 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7081 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7141 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     7201 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7261 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7321 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7381 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7441 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7501 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7561 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7621 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7681 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7741 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7801 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     7861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10021 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10081 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10141 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10201 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10261 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10321 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10381 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10441 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
