LOCUS       sequence-120712-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SP2
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttccttcct gcagggtacc acgcgtaccc aacacacgga cacatgcacg aacacgcatg
       61 cagagagatc atacatcatc tatagagaag tacacgtaaa agtaagactc gtcgtctgcc
      121 agctacaacc gagaaggcga cgtgaaagga acaagaaagg aggaggaggg cggccgctta
      181 caccactttg tacaagaaag ttgggtacaa gttcttcgtg accaggtggg tcttgtaatg
      241 cttggtgagg tggtcactcc tcatgaagcg cttctgacac tgggcgcact cgaagcgttt
      301 gtcccctgtg tgggtgcgag catgccgttg gagctcgtca ctccgtgtga acctcttccc
      361 acagaagaac cagttgcaga caaagggccg ctcgccagtg tgcaggcgca catgggcacg
      421 cagcaaggac gtcttacgga acgtcttgcc acagtcgggg atgtggcaca cgtgcttctt
      481 cttgccctgc tctccagacc tcttctcccc atccttgcag ttgggacacg tgcaggccat
      541 gcggcgccgc ttctccccgg gctgggtctc tccggccagg gcttgttcca tttgcagctg
      601 gatctgggtg gggctcagcc cactgatggt caggttgttc ccagaaacat tctgcactgt
      661 cagctgctgc tgcccgcctg tgttggtgat ggtgacaggc acgccctgga cctggacacc
      721 attgatgttg atggtctgca ttgcctgggc agctgccgcc aactgggctg cattcaggct
      781 gatgatgctc ccggctgggg caatctttgg caggggacgc tcttttcgga gaattgcagc
      841 tgagtgcttt ttgctggtcc cactcagatg gggagcacgg gatgcagggc tgctacaggt
      901 ggtgttagag gtggctgcag ctgttgctgg ggggctgtcc tggacaagga ctgtctgcac
      961 ctcaccggaa ggcgtgcgga tgtagacctg agtaggtgtc ggctcagctg cctggatctg
     1021 aaagttctgg gagggcttct ggggtacagt ggggagggtg gcaaa
//
