LOCUS       sequence-120718-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SP2
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..50
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    51..75
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctcggcat ggacgagcta tacaagctgg aggtgctctt ccagggtccg acaagtttgt
       61 acaaaaaagt tggcatggct gccactgctg ctgtgagtcc cagtgactac ctgcagcctg
      121 ccgcctccac cacccaggac tcccagccat ctcccttagc cctgcttgct gcaacatgta
      181 gcaaaattgg ccctccagca gttgaagctg ctgtgacacc tcctgctccc ccacagccca
      241 caccgcggaa acttgtccct atcaaacctg cccctctccc tctcagcccc ggcaagaata
      301 gctttggaat cttgtcctcc aaaggaaata tacttcagat tcaggggtca caactgagcg
      361 cctcctatcc tggagggcag ctggtgttcg ctatccagaa tcccaccatg atcaacaaag
      421 ggacccgatc aaatgccaat atccagtacc aggcggtccc tcagattcag gcaagcaatt
      481 cccaaaccat ccaagtacag cccaatctca ccaaccagat ccagatcatc cctggcacca
      541 accaagccat catcaccccc tcaccgtcca gtcacaagcc tgtccccatc aagccagccc
      601 ccatccagaa gtcgagtacg accaccaccc ccgtgcagag cggggccaat gtggtgaagt
      661 tgacaggtgg gggcggcaat gtgacgctca ctctgcccgt caacaacctc gtgaacgcca
      721 gtgacaccgg ggcccctact cagctcctca ctgaaagccc cccaaccccg ctgtctaaga
      781 ctaacaagaa agcaaggaag aagagccttc ctgcctccca gccccctgtg gctgtggctg
      841 agcaggtgga gacggtgctg atcgagacca ccgcggacaa catcatccag gcaggaaata
      901 acctgctcat tgttcagagc cctggtgggg gccagccagc tgtggtccag caggtccagg
      961 tggtgccccc caaggccgag cagcagcagg tggtacagat cccccagcag gctctgcggg
     1021 tggtgcaggc ggcatctgcc accctcccca ctgtacccca gaagccctcc cagaactttc
     1081 agatccaggc agctgagccg acacctactc aggt
//
