LOCUS       sequence-120719-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SP2
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    186..210
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tncttccttc tgcaggggta ccacgcgnta cccaacacac ggnacacatg cacgaacacg
       61 catgcagaga gatcatacat catctataga gaagtacacg taaaagtaag actcgtcgtc
      121 tgccagctac aaccgagaag gcgacgtgaa aggaacaaga aaggaggagg agggcggccg
      181 cttacaccac tttgtacaag aaagttgggt acaagttctt cgtgaccagg tgggtcttgt
      241 aatgcttggt gaggtggtca ctcctcatga agcgcttctg acactgggcg cactcgaagc
      301 gtttgtcccc tgtgtgggtg cgagcatgcc gttggagctc gtcactccgt gtgaacctct
      361 tcccacagaa gaaccagttg cagacaaagg gccgctcgcc agtgtgcagg cgcacatggg
      421 cacgcagcaa ggacgtctta cggaacgtct tgccacagtc ggggatgtgg cacacgtgct
      481 tcttcttgcc ctgctctcca gacctcttct ccccatcctt gcagttggga cacgtgcagg
      541 ccatgcggcg ccgcttctcc ccgggctggg tctctccggc cagggcttgt tccatttgca
      601 gctggatctg ggtggggctc agcccactga tggtcaggtt gttcccagaa acattctgca
      661 ctgtcagctg ctgctgcccg cctgtgttgg tgatggtgac aggcacgccc tggacctgga
      721 caccattgat gttgatggtc tgcattgcct gggcagctgc cgccaactgg gctgcattca
      781 ggctgatgat gctcccggct ggggcaatct ttggcagggg acgctctttt cggagaattg
      841 cagctgagtg ctttttgctg gtcccactca gatggggagc acgggatgca gggctgctac
      901 aggtggtgtt agaggtggct gcagctgttg ctggggggct gtcctggaca aggactgtct
      961 gcacctcacc ggaaggcgtg cggatgtaga cctgagtagg tgtcggctca gctgcctgga
     1021 tctgaaagtt ctgggagggc ttctggggta cagtggggag ggtggcagat gccgcctgc
//
