LOCUS       sequence-120720-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner
            for enChIP analysis to purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     An enChIP system for the analysis of bacterial genome functions.
  JOURNAL   BMC Res Notes. 2018 Jun 14;11(1):387. doi: 
            10.1186/s13104-018-3486-3.
  PUBMED    29898790
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2018-06-14"; volume: "11(1):387. doi"; pages: " 
            10.1186/s13104-018-3486-3"
COMMENT     Sequence Label: 3xFLAG-dCas9/p-bacteria
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(16..62)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
       61 tgatgcctgg agatccttac tcgagttagt cacctcctag ctgactcaaa tcaatgcgtg
      121 tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact tcttttgtag
      181 acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg ggagctccaa
      241 gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt ggtttgtctc
      301 tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa ataacacgct
      361 tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc tgctccacaa
      421 acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa tgactagcta
      481 aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt tgtaattctc
      541 cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta tatttaggta
      601 gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa aagtcaatcg
      661 gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct ttaacggatt
      721 ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa taagctaccg
      781 ttggactatc aaaaccacca tatttttttg gatcccagtc ttttttacga gcaataagct
      841 tgtccgaatt tctttttggt aaaattgact ccttggagaa tccgcctgtc tgtacttctg
      901 ttttcttgac aatattgact tggggcatgg acaatacttt gcgcactgtg gcaaaatctc
      961 gccctttatc ccagacaatt tctccagttt ccccattagt ttcgattaga gggcgtttgc
     1021 gaatctctcc atttgcaagt gtaatttctg ttttgaagaa gt
//
