LOCUS       sequence-120721-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner
            for enChIP analysis to purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     An enChIP system for the analysis of bacterial genome functions.
  JOURNAL   BMC Res Notes. 2018 Jun 14;11(1):387. doi: 
            10.1186/s13104-018-3486-3.
  PUBMED    29898790
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2018-06-14"; volume: "11(1):387. doi"; pages: " 
            10.1186/s13104-018-3486-3"
COMMENT     Sequence Label: 3xFLAG-dCas9/p-bacteria
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agttcactca aacctccacg ttcagctttc gttaaattat caaacttacg ttgagtgatt
       61 aacttggcgt ttagaagttg tctccaatag tttttcatct ttttgactac ttcttcactt
      121 ggaacgttat ccgatttacc acgattttta tcagaacgcg ttaagacctt attgtctatt
      181 gaatcgtctt taaggaaact ttgtggaaca atggcatcga catcataatc acttaaacga
      241 ttaatatcta attcttggtc cacatacatg tctcttccat tttggagata atagagatag
      301 agcttttcat tttgcaattg agtattttca acaggatgct ctttaagaat ctgacttcct
      361 aattctttga taccttcttc gattcgtttc atacgctctc gcgaattttt ctggcccttt
      421 tgagttgtct gattttcacg tgccatttca ataacgatat tttctggctt atgccgcccc
      481 attactttga ccaattcatc aacaactttt acagtctgta aaataccttt tttaatagca
      541 gggctaccag ctaaatttgc aatatgttca tgtaaactat cgccttgtcc agacacttgt
      601 gctttttgaa tgtcttcttt aaatgtcaaa ctatcatcat ggatcagctg cataaaattg
      661 cgattggcaa aaccatctga tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc
      721 ctaataccat taatcaattt tcgagacaaa cgtccccaac cagtataacg gcgacgttta
      781 agctgtttca tcaccttatc atcaaagagg tgagcatatg ttttaagtct ttcctcaatc
      841 atctccctat cttcaaataa ggtcaatgtt aaaacaatat cctctaagat atcttcattt
      901 tcttcattat ccaaaaa
//
