LOCUS       sequence-120723-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner
            for enChIP analysis to purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     An enChIP system for the analysis of bacterial genome functions.
  JOURNAL   BMC Res Notes. 2018 Jun 14;11(1):387. doi: 
            10.1186/s13104-018-3486-3.
  PUBMED    29898790
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2018-06-14"; volume: "11(1):387. doi"; pages: " 
            10.1186/s13104-018-3486-3"
COMMENT     Sequence Label: 3xFLAG-dCas9/p-bacteria
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..32
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             42..107
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gaaaagaatt caaaagatct aaagaggaga aaggatctat ggactacaaa gaccatgacg
       61 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctc atggataaga
      121 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
      181 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
      241 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
      301 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
      361 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
      421 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
      481 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
      541 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
      601 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
      661 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
      721 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
      781 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
      841 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
      901 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
      961 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     1021 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     1081 gctacgatga acatcatca
//
