LOCUS       sequence-120727-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vanillate-inducible gene expression plasmid for Sphingomonas melonis
            Fr1; destination vector for Gateway cloning, allowing C-terminal 
            fusion of proteins to SYFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Synthetic vanillate-regulated promoter for graded gene expression in
            Sphingomonas.
  JOURNAL   Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453.
  PUBMED    25262659
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-29"; volume: "4:6453. doi"; pages: " 
            10.1038/srep06453"
COMMENT     Sequence Label: pVYD
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cgggcgaagc actgcacgcc gtagcccagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgcggaagct
      301 tcctctacta gtattggatc caatttagcg gcttcgcggc gatccgtcaa gattggatcc
      361 aatgttaact ccggtacatt gacatatgta tccgctcatg atacaataac cctgatgcta
      421 gcagggagag accccgaatg gacatgccgc gcataaagcc gggccagcgc gtgatgatgg
      481 ctcttcgcaa gatgatcgcc tcgggcgaga tcaagagcgg cgagcgtatc gccgaaatcc
      541 cgacagcggc ggcgctgggc gtctcgcgca tgcccgtgcg cacggccttg cggagtctgg
      601 agcaggaagg gctcgtggtg cggctgggcg cgcggggtta tgccgcccga ggcgtgtcca
      661 gcgaccagat ccgcgacgcc atcgaggtgc gaggggtgct ggagggcttt gcggcccggc
      721 gtctggccga gcgcggaatg acggcggaga cccacgcgcg gttcgtcgcc ttgatcgccg
      781 agggcgaagc gctgttcgcg gctgggcggc ttaacggcga ggacctggat cgctacgccg
      841 cgtacaatca ggccttccat gacacgctgg tcagcgctgc gggcaacggc gctgtcgaga
      901 gcgccctggc gcgcaatggt ttcgagccgt tcgcggcggc cggtgcgctg gccctggatc
      961 tgatggactt gccggccgag tacgaacacc ttctggccgc ccatcgccag catca
//
