LOCUS       sequence-120729-       10214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vanillate-inducible gene expression plasmid for Sphingomonas melonis
            Fr1; destination vector for Gateway cloning, allowing C-terminal 
            fusion of proteins to HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Synthetic vanillate-regulated promoter for graded gene expression in
            Sphingomonas.
  JOURNAL   Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453.
  PUBMED    25262659
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-29"; volume: "4:6453. doi"; pages: " 
            10.1038/srep06453"
COMMENT     Sequence Label: pVHD
FEATURES             Location/Qualifiers
     source          1..10214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2641..2667
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2686..2810
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2835..2840,2841..2858,2859..2865)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2919..3578
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2985..3004)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3631..4219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4373..4390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             4852..5157
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5036..5055
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5198..5322)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5364..5429
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5501..5518)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5501..5517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5510..5532)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5726..5745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5845..5867
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5905..5923)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             6709..7908
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(8139..9287)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9559..9930)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9963..10072
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttttcag tcggcgcgaa tgctccacgc cgcgcccagc ggcgcgccgg
     1801 cgctcgccgc ggcctcgaag accttggcgt tacgaatggc ggccagggca tggtcgcgca
     1861 tgatccgctc ggccccctcg gcgtcgccgc acgagaccgc gtccagcacc gcctgatgct
     1921 ggcgatgggc ggccagaagg tgttcgtact cggccggcaa gtccatcaga tccagggcca
     1981 gcgcaccggc cgccgcgaac ggctcgaaac cattgcgcgc cagggcgctc tcgacagcgc
     2041 cgttgcccgc agcgctgacc agcgtgtcat ggaaggcctg attgtacgcg gcgtagcgat
     2101 ccaggtcctc gccgttaagc cgcccagccg cgaacagcgc ttcgccctcg gcgatcaagg
     2161 cgacgaaccg cgcgtgggtc tccgccgtca ttccgcgctc ggccagacgc cgggccgcaa
     2221 agccctccag cacccctcgc acctcgatgg cgtcgcggat ctggtcgctg gacacgcctc
     2281 gggcggcata accccgcgcg cccagccgca ccacgagccc ttcctgctcc agactccgca
     2341 aggccgtgcg cacgggcatg cgcgagacgc ccagcgccgc cgctgtcggg atttcggcga
     2401 tacgctcgcc gctcttgatc tcgcccgagg cgatcatctt gcgaagagcc atcatcacgc
     2461 gctggcccgg ctttatgcgc ggcatgtcca ttcggggtct ctccctgcta gcatcagggt
     2521 tattgtatca tgagcggata catatgtcaa tgtaccggag ttaacattgg atccaatctt
     2581 gacggatcgc cgcgaagccg ctaaattgga tccaatacta gtagaggaag cttccgcatg
     2641 tacccgtacg acgtcccgga ctacgccatt aatatgactc tagagacaag tttgtacaaa
     2701 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     2761 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     2821 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     2881 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     2941 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     3001 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     3061 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     3121 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     3181 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     3241 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     3301 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     3361 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     3421 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     3481 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     3541 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtcgttcc actgagcgtc
     3601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4381 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4441 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4501 cgcaattaat acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     4561 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     4621 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     4681 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     4741 gtcgtccgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     4801 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     4861 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     4921 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     4981 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     5041 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     5101 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     5161 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     5221 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     5281 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatcgc tagctagagg
     5341 taccgagctc caattgggat ccggactaca aggaccacga cggcgactat aaggaccacg
     5401 acatcgacta caaggatgac gacgacaagt gaggatccgg tgaagtgacc cgcaaaaaaa
     5461 aaggccaggg gatcgctccc ccggcctttt ttttgaattc actggccgtc gttttacaac
     5521 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5581 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5641 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5701 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5761 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5821 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5881 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5941 tgataataat ggtttcttag caccctttct cggtccttca acgttcctga caacgagcct
     6001 ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc
     6061 ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac
     6121 ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca cggccccaac
     6181 agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc
     6241 gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc
     6301 ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt
     6361 cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg tatgattctc
     6421 cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta
     6481 aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac
     6541 gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct gcaactttgt
     6601 catgattgac actttatcac tgataaacat aatatgtcca ccaacttatc agtgataaag
     6661 aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt gaaacccaac
     6721 atacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt
     6781 atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt caccgcccac
     6841 tatggcattc tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc
     6901 gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctcgctggc cggcgccact
     6961 gtcgactacg ccatcatggc gacagcgcct ttcctttggg ttctctatat cgggcggatc
     7021 gtggccggca tcaccggggc gactggggcg gtagccggcg cttatattgc cgatatcact
     7081 gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg
     7141 gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct ccccccacgc tccgttcttc
     7201 gccgcggcag ccttgaacgg cctcaatttc ctgacgggct gtttcctttt gccggagtcg
     7261 cacaaaggcg aacgccggcc gttacgccgg gaggctctca acccgctcgc ttcgttccgg
     7321 tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg tcttcttcat catgcaactt
     7381 gtcggacagg tgccggccgc gctttgggtc attttcggcg aggatcgctt tcactgggac
     7441 gcgaccacga tcggcatttc gcttgccgca tttggcattc tgcattcact cgcccaggca
     7501 atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc gggcactcat gctcggaatg
     7561 attgccgacg gcacaggcta catcctgctt gccttcgcga cacggggatg gatggcgttc
     7621 ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc cggcgctgca agcaatgttg
     7681 tccaggcagg tggatgagga acgtcagggg cagctgcaag gctcactggc ggcgctcacc
     7741 agcctgacct cgatcgtcgg acccctcctc ttcacggcga tctatgcggc ttctataaca
     7801 acgtggaacg ggtgggcatg gattgcaggc gctgccctct acttgctctg cctgccggcg
     7861 ctgcgtcgcg ggctttggag cggcgcaggg caacgagccg atcgctgatc gtggaaacga
     7921 taggcctatg ccatgcgggt caaggcgact tccggcaagc tatacgcgcc ctagaattgt
     7981 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
     8041 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
     8101 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
     8161 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
     8221 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
     8281 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
     8341 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
     8401 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
     8461 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
     8521 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
     8581 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
     8641 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
     8701 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
     8761 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
     8821 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
     8881 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
     8941 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
     9001 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
     9061 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
     9121 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
     9181 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
     9241 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
     9301 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
     9361 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
     9421 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag ctccgcgaag
     9481 tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg
     9541 ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt
     9601 gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc
     9661 gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc
     9721 caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat
     9781 tttgtagccc tggccgacgg ccagcaggta ggcctacagg ctcatgccgg ccgccgccgc
     9841 cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc
     9901 cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
     9961 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
    10021 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
    10081 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
    10141 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
    10201 ggaaaagatc cgtc
//
