LOCUS       sequence-120730-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag 
            for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pRAV23
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      295..342
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(361..377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(361..377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(374..396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    385..401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(409..415,416..433,434..439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    454..475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(592..609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(843..862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattctaata cgactcacta tagggtcgct caaacattaa gtggtgttgt gcgaaaagaa
       61 tctcgaagac aagccggcat agcgcccgaa ctaccggtac cggtagttga cgaggatgga
      121 ggttatcgaa ttttcggcgg atgcctcccg gctgagtgtg cagatcacag ccgtaaggat
      181 ttcttcaaac caagggggtg actccttgaa caaagagaaa tcacatgatc ttcctttttt
      241 ctagacgtac accatcaggg tacgtttttc agacaccatc agggtctggg atccctagca
      301 taaccccttg gggcctctaa acgggtcttg aggggttttt tgaagcttgg cgtaatcatg
      361 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      421 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      481 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      541 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      661 aatacggtta tccacagaat cgggggataa cgcaggaaag aacatgtgag caaaaggcca
      721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1081 cccggtaaga
//
