LOCUS       sequence-120732-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag 
            for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pRAV23
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(23..70)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(340..359)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(341..359)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(366..382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(591..610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     710..732
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(770..788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        856..960
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgaccatgat tacgccaagc ttcaaaaaac ccctcaagac ccgtttagag gccccaaggg
       61 gttatgctag ggatcccaga ccctgatggt gtctgaaaaa cgtaccctga tggtgtacgt
      121 ctagaaaaaa ggaagatcat gtgatttctc tttgttcaag gagtcacccc cttggtttga
      181 agaaatcctt acggctgtga tctgcacact cagccgggag gcatccgccg aaaattcgat
      241 aacctccatc ctcgtcaact accggtaccg gtagttcggg cgctatgccg gcttgtcttc
      301 gagattcttt tcgcacaaca ccacttaatg tttgagcgac cctatagtga gtcgtattag
      361 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      421 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
      541 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      601 tctgatgccg catagttaag ccagccccga cacccgccaa cacccactga cgcgccctga
      661 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      721 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
      781 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctga
//
