LOCUS       sequence-120746-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of TRMT10C, full coding sequence (mitoch. form)
            + N-term. His tag (thrombin site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Holzmann J, Frank P, Loffler E, Bennett KL, Gerner C, Rossmanith W
  TITLE     RNase P without RNA: identification and functional reconstitution of
            the human mitochondrial tRNA processing enzyme.
  JOURNAL   Cell. 2008 Oct 31;135(3):462-74. doi: 10.1016/j.cell.2008.09.013.
  PUBMED    18984158
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.09"; journalName: "Cell"; date: "2008-10-31- 
            31"; volume: "135"; issue: "3"; pages: "462-74"
COMMENT     Sequence Label: pET28-MRPP1
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg tcttccaaaa
      121 taccagctgt tacttatcct aaaaatgaga gtacaccccc ttctgaagag ctagagttgg
      181 ataagtggaa aactaccatg aaatctagtg tgcaagaaga atgtgtttca acaatctcaa
      241 gcagtaagga tgaagatcct ctagctgcca ccagagagtt cattgagatg tggagattgc
      301 ttggcagaga agtaccagaa cacatcactg aagaagagct caaaaccctt atggaatgtg
      361 tttctaacac agcaaaaaaa aaatatttaa aatatttata tacgaaggaa aaagtgaaaa
      421 aagctaggca aataaaaaag gaaatgaaag cagcagcaag ggaagaagca aaaaatatca
      481 agctgctaga aaccactgag gaagataaac agaaaaactt tctattttta cgactttggg
      541 ataggaatat ggacatagca atgggctgga agggtgccca ggccatgcag tttggacaac
      601 ctttggtttt tgacatggct tacgaaaatt atatgaaacg aaaagaattg cagaatactg
      661 tttcccagct tttagaaagt gaaggatgga acagaagaaa tgttgatcct ttccatattt
      721 atttctgcaa tctaaaaata gatggtgctt tgcacagaga gttagttaaa cggtatcaag
      781 aaaaatggga caaattgctt ttaacatcaa cagaaaagtc tcatgtagat ttatttccaa
      841 aggacagtat tatctattta actgcagatt ctcccaatgt tatgactact ttcaggcatg
      901 acaaagttta tgtaattggg tcttttgttg ataagagtat gcagccaggc acatccctag
      961 ccaaggcaaa acggctgaac ctggcaactg aatgccttcc attagataaa tatttacaat
     1021 gggaaattgg taacaaaaat ctcaccttag atcaaatgat acgtattttg ttatgtctga
     1081 aaaacaatgg taattggcaa gaggctctgc aattcgttcc caagagaaaa catactggtt
     1141 tt
//
