LOCUS       sequence-120748-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ZFYVE9
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gttgttttcc agaatataaa tgatgagttc catgacaacg gggccctcac
      241 taagctggca ggcccctcca tggatcaccg gcaccaaggc gctgtccaga tcattcatgt
      301 actgcgaggg aaggggctgg ccattgctcc ctgcttgata gccaacctga tctgagtcaa
      361 gtgtcacacg tagtcccagt ttggtcattc catcttcctt cagaagtttc aggtgaggac
      421 agagagcaag gcaaaaagct ttggcaacat gctcagtcaa tctactgtga tctgcaggat
      481 cactgaggca attgtgctgg tcatcgtttt ctaggaaaaa cacctctgtc catctgatta
      541 cttttccatt tgctttatat tctgatccat ggaatatctt cacatttgtt atagtctcca
      601 tggacttccc atctatagga cttacgacac ccttgctaac gttcttgtca tcatccaccc
      661 actggatgtg gatgtgctcc tggggttcct ccgcgtccgc cttcccacag gtgatggtga
      721 agtccttcat ctctcgcagt gcctgcctca aggaatccat gttctctgca gtaatctgga
      781 ccataacacc atcttccaca atactggact tggcaaggta tccagaagag gatttcagag
      841 cgccactgaa cacaaagaaa ctggcaccag tcacttttct gggctgattg tgaatactga
      901 tagcctgggt ctgatagttt ccatcatcat tctgtacaca cacaagatga gagtctgcct
      961 tt
//
