LOCUS       sequence-120751-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2H1
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagctgg gtccaacgtt ttcttcatca gacgccgtga ctgccatgtg tggagcttgt
      241 tgtaggctgt ctggagcatc tcttctatgt gacttaccaa atttgtgctt aaatactgtc
      301 tccgaatctt ttcttggaat ggacagagct tcgtaacttg gaatcgttcc aaattacttt
      361 tcattttcac taccttttct tctaggaatg gcgtattaac aggaaagcag gaccagaaat
      421 gtcgtagaag ttctccaaca gctacatata agtgtttcaa ttcagattga atatcatttg
      481 gcaccatctg gtttatggct tgctgtgttc ctccctgcat aagtgcccct ccaggtgaca
      541 gtgctgtgat ggtactactg gcagcactac ttgagagaac ctgagttaac ttgggtgtat
      601 aagcttccat ttcttgtcta atactttgaa aagaattaat aatgtcctga cttgttgcat
      661 actgtagtga ctggattgga gttggaccat gataatacct atctgacttc ttgaggttta
      721 gtgcaatcgt ttttacagaa ttatttttcc ccaagtcttc atattcaatg gactcttgta
      781 atttcgccct tttgactgct ggctgaaagc agtctgcatc tccggaattt ccatccatgt
      841 tgctgggctc actagtttgt tcattttgtg c
//
