LOCUS       sequence-120752-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of SDR5C1 (HSD17B10), full coding sequence + 
            N-term. His tag (thrombin site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Holzmann J, Frank P, Loffler E, Bennett KL, Gerner C, Rossmanith W
  TITLE     RNase P without RNA: identification and functional reconstitution of
            the human mitochondrial tRNA processing enzyme.
  JOURNAL   Cell. 2008 Oct 31;135(3):462-74. doi: 10.1016/j.cell.2008.09.013.
  PUBMED    18984158
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.09"; journalName: "Cell"; date: "2008-10-31- 
            31"; volume: "135"; issue: "3"; pages: "462-74"
COMMENT     Sequence Label: pET28-HADH2
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             900..917
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(970..988)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      984..1031
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gcagcagcgt
      121 gtcggagcgt gaagggcctg gtggcggtaa taaccggagg agcctcgggc ctgggcctgg
      181 ccacggcgga gcgacttgtg gggcagggag cctctgctgt gcttctggac ctgcccaact
      241 cgggtgggga ggcccaagcc aagaagttag gaaacaactg cgttttcgcc ccagccgacg
      301 tgacctctga gaaggatgtg caaacagctc tggctctagc aaaaggaaag tttggccgtg
      361 tggatgtagc tgtcaactgt gcaggcatcg cggtggctag caagacgtac aacttaaaga
      421 agggccagac ccataccttg gaagacttcc agcgagttct tgatgtgaat ctcatgggca
      481 ccttcaatgt gatccgcctg gtggctggtg agatgggcca gaatgaacca gaccagggag
      541 gccaacgtgg ggtcatcatc aacactgcca gtgtggctgc cttcgagggt caggttggac
      601 aagctgcata ctctgcttcc aaggggggaa tagtgggcat gacactgccc attgctcggg
      661 atctggctcc cataggtatc cgggtgatga ccattgcccc aggtctgttt ggcaccccac
      721 tgctgaccag cctcccagag aaagtgtgca acttcttggc cagccaagtg cccttcccta
      781 gccgactggg tgaccctgct gagtatgctc acctcgtaca ggccatcatc gagaacccat
      841 tcctcaatgg agaggtcatc cggctggatg gggccattcg tatgcagcct tgactcgagc
      901 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      961 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     1021 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     1081 cggcgcat
//
