LOCUS       sequence-120753-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2H1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgacttcag ctggaggtgc tcttccgggt ccgacaagtt tgtacaaaaa agcaggctcc
       61 accatggcaa cctcatctga agaagttttg ctgattgtaa agaaagtgcg tcaaaagaag
      121 caggatggag ctctgtacct catggcagaa agaattgctt gggcacctga aggcaaagat
      181 agatttacaa tcagccatat gtatgcagat attaaatgcc agaaaattag tccagaagga
      241 aaagctaaaa ttcagcttca gctggtccta catgcagggg acacaactaa cttccatttt
      301 tccaatgaaa gcacagcagt gaaagagcga gatgcagtaa aagaccttct tcagcagctg
      361 ctgcccaaat tcaagaggaa agcaaataaa gaactggaag agaagaacag aatgctgcaa
      421 gaagatcctg ttttgtttca gctttataaa gaccttgttg tgagtcaagt gatcagtgct
      481 gaggaattct gggccaatcg tttaaatgtg aatgcaacag atagttcttc cacatccaat
      541 cataagcagg atgttggcat ttctgctgca tttctggctg atgtccggcc ccaaactgat
      601 ggctgtaacg gtctaagata taatttaact tctgatatca ttgagtccat atttaggacc
      661 tatccagcag taaaaatgaa atatgcagaa aatgttcccc acaacatgac agagaaggaa
      721 ttctggacac gttttttcca gtcccattat tttcacaggg atcggctgaa tacagggtca
      781 aaggatctct ttgcagaatg
//
