LOCUS       sequence-120756-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PRORP (KIAA0391), full coding sequence 
            (mitoch. form) + C-term. myc-His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Holzmann J, Frank P, Loffler E, Bennett KL, Gerner C, Rossmanith W
  TITLE     RNase P without RNA: identification and functional reconstitution of
            the human mitochondrial tRNA processing enzyme.
  JOURNAL   Cell. 2008 Oct 31;135(3):462-74. doi: 10.1016/j.cell.2008.09.013.
  PUBMED    18984158
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.09"; journalName: "Cell"; date: "2008-10-31- 
            31"; volume: "135"; issue: "3"; pages: "462-74"
COMMENT     Sequence Label: pET28-MRPP3-myc-His
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(129..146)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(162..191)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tcaggcacag tcgaggctga tcagcgggtt
      121 taaattcaat gatgatgatg atgatggtcg acggcgctat tcagatcctc ttctgagatg
      181 agtttttgtt cgggccctct agatgtcttt tggtggaggc aaagccattt ggttggtact
      241 tcacaggaac atctttctac caagtcttca tcatatggta tgtgccacga gtctccagtt
      301 gtttgcacca ctgtgtcata gctgagaata cgctgaaagg ttagttttga tcctggaaac
      361 ctatttacaa ttgccagctg atgtccctgc tgccacttaa aaaacaggcg ttgggtcttg
      421 gcatcaggca gacaggcctt gtggtcccgc atcaggtctc ttgtgataaa cctgcagtga
      481 ttcccggagt gcagtgtggc atacagaagg aatggatcat cctccgagat gtcatcagca
      541 aaaaaacagc tggcttgctt ttgcacctct tccatctcat cccgactcca ctgggaactc
      601 cgtcttagca tgtgcttccg gcctaggacc agcagtcgca gattccgttt ggctagttga
      661 gagacgacat tcaagagaag ttgagattca cgaactttag gaaacatttt ggcaacattg
      721 agaccatcaa tgacaacatc aaaaggagga cgagatttta tgaagttctc aaatctctta
      781 agttcctgag gtgttgtctt tctgtactgg tcacctccat ctatcacatc cctcatgatt
      841 tttcccttaa gacattcata ttcttctgga ctcagctgaa tagactctat ggtttttcca
      901 cagcccgaac actggccact ttttcggact gtggtgaatt gtcctttcca ttgttttcca
      961 ggaacactct caaaccatgt ttttatactg tgtgcaaatg actcccctgg atacagctga
     1021 ttatttctta gatatgaaag aatatctagt agtttatttg aatagttatc atcctttatg
     1081 tc
//
