LOCUS       sequence-120758-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for GFP-tagged alpha2-chimaerin (GFP at 
            N-terminus of alpha2-chimaerin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Beg AA, Sommer JE, Martin JH, Scheiffele P
  TITLE     alpha2-Chimaerin is an essential EphA4 effector in the assembly of 
            neuronal locomotor circuits.
  JOURNAL   Neuron. 2007 Sep 6;55(5):768-78.
  PUBMED    17785183
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2007-09-6"; volume: "55(5)"; pages: "768-78"
COMMENT     Sequence Label: pEGFP-C1-alpha2-chimaerin
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(26..28,29..31,32..742)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(26..28,29..31,32..742)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(332..351)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     679..700
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
       61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      181 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      241 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      481 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
      781 agtcgacatg gctctgaccc tgttcgatac agatgaatat agacctcctg tttggaaatc
      841 ttacttatac cagctgcagc aggaagcc
//
