LOCUS       sequence-120763-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound 
            Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 140_pSKB3-GBD-LUMP
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg cctaccattc
       61 atttaaaatt tcaacgttca ctttcacatc ttttgctaac gatgcaatgt tagtactcgc
      121 taacaaatca cgcgagtaat tcaatgtaat aatgctgtct gacatatcag tgataggcaa
      181 agagatccca tcaattccaa tatcatcttt cactgttaag ttttcagcta accctttagg
      241 gatatcaatc agtacagaaa aacctgcgtc attctcttct attgctgcga ccagcgcagt
      301 gcctttaata tttcctgtta atccaccacg gccaaccact tcaccaaatt tagggtggat
      361 ctctaggttt acctgatcgc cttcttttaa tccatcaaat gttgtcgtac ccaatgcttg
      421 gtcgatatca aagtacacca tatcgcctaa aatacggaca actgtcaatg aacaaccatt
      481 tactaacatt acagtatcta catcaacaag ttgaaacata ccctcaggga atgcaatccc
      541 atgtcgctga ctatcttcac tttttgaaat ggatcgaata acaccacgac cttgaacaat
      601 acctctaaag ctagcgctgc cgctgccgtt gcccaggtac agctcgtcga tgcggtccgg
      661 aaagccccag caatggcgag gatcgctgtc gccgtggccg gtatggatga agctgttctg
      721 cagcggctgg ctgatgtcc
//
