LOCUS       sequence-120765-        1168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PRORP1 (At2g32230), full coding sequence 
            (mitoch./chloropl. form) + C-term. His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Pavlova LV, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann 
            RK
  TITLE     tRNA processing by protein-only versus RNA-based RNase P: kinetic 
            analysis reveals mechanistic differences.
  JOURNAL   Chembiochem. 2012 Oct 15;13(15):2270-6. doi: 10.1002/cbic.201200434.
            Epub 2012 Sep 13.
  PUBMED    22976545
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201200434"; journalName: "Chembiochem"; date: 
            "2012-10-15- 15"; volume: "13"; issue: "15"; pages: "2270-6"
COMMENT     Sequence Label: pET28-At_PRORP1-His
FEATURES             Location/Qualifiers
     source          1..1168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcccat
       61 ttcgtttcta ccatacttcc ttacttgtga aaggcaccag agatagacgt cttattcttg
      121 ttgagcgtag cagacatcta tgtacattgc ctctcgctgc tgctaaacaa tcagcagctt
      181 ctccttctga aaacttatcg aggaaagcga agaagaaagc tattcaacag tctcctgaag
      241 cattgctgaa acaaaagcta gatatgtgct ctaagaaagg tgatgtcttg gaagctcttc
      301 gcttgtatga tgaggcaaga cgcaatggcg tgcagcttag ccagtaccat tataatgtgt
      361 tgctttatgt atgctcgttg gctgaggcag caacagagtc gtctccaaat ccagggctta
      421 gtaggggttt tgatattttt aagcagatga ttgttgataa agttgttccc aatgaagcta
      481 catttacaaa cggtgctaga ctcgcagttg caaaggatga cccggaaatg gctttcgata
      541 tggtgaaaca gatgaaagcc tttggtattc aaccaaggtt gagatcctat ggacctgccc
      601 tttttggttt ctgtaggaaa ggtgatgcag acaaagctta tgaagtcgat gcgcatatgg
      661 ttgaatcaga ggtagtccct gaagaacctg agcttgctgc tcttttgaaa gttagtatgg
      721 atacaaagaa cgcagacaag gtctataaga cgttgcagcg attgagagat ttggtgagac
      781 aggtttctaa atcgactttt gatatgattg aggagtggtt taaatctgaa gtagctacaa
      841 aaactggagt gaagaaatgg gatgtgaaga aaataaggga tgcggttgtg agtggtggtg
      901 gtgggtggca tggacaagga tggctgggga ctggcaaatg gaatgtgaaa agaacagaga
      961 tggatgagaa tggtgtatgt aaatgttgca aagagaagct tgtttgtatt gatatcaatc
     1021 cggttgagac agaaaccttt gctgcatcgt tgacccgatt ggcatgtgaa agagaagtca
     1081 aggctaattt taatcagttt caggaatggc tggaaagaca tggaccgttt gatgcagtca
     1141 ttgatggagc caacatgggc ttagtcaa
//
