LOCUS       sequence-120767-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PRORP1 (At2g32230), full coding sequence 
            (mitoch./chloropl. form) + C-term. His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Pavlova LV, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann 
            RK
  TITLE     tRNA processing by protein-only versus RNA-based RNase P: kinetic 
            analysis reveals mechanistic differences.
  JOURNAL   Chembiochem. 2012 Oct 15;13(15):2270-6. doi: 10.1002/cbic.201200434.
            Epub 2012 Sep 13.
  PUBMED    22976545
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201200434"; journalName: "Chembiochem"; date: 
            "2012-10-15- 15"; volume: "13"; issue: "15"; pages: "2270-6"
COMMENT     Sequence Label: pET28-At_PRORP1-His
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agaggtgttt
       61 tggatctttt tgcgcataac cattgccttg atgtttggag atcatcttct acgctcattg
      121 gtacatgcca ggttccatct tcagactcct gtataacaat ggagtatgga ggtggcatat
      181 taagtttgag tccatcttct ctagtgacag agatccgaac ctgatgcttc tctttccacc
      241 ttgggaagaa actatttcca agtaactgga agagatggtc tctcatctca tcgtttgtta
      301 ctaacaaaca tttgcaactt accgcagcgt aaagccaata ccaatcatcg tttgagccag
      361 gaggggtagc atagagagca ccagcgtttt tccatttctc tagcaaggct ctgtttttag
      421 ggtatgtagc tggacctcca tttacacgac tcttgtgcag gatcacaagt ggtaaccttt
      481 tcgacgggct tatttgttgg catctttgga cagtgttgtt aagctggaag aagctgaaac
      541 tgcgctggtt gactaagccc atgttggctc catcaatgac tgcatcaaac ggtccatgtc
      601 tttccagcca ttcctgaaac tgattaaaat tagccttgac ttctctttca catgccaatc
      661 gggtcaacga tgcagcaaag gtttctgtct caaccggatt gatatcaata caaacaagct
      721 tctctttgca acatttacat acaccattct catccatctc tgttcttttc acattccatt
      781 tgccagtccc cagccatcct tgtccatgcc acccaccacc accactcaca accgcatccc
      841 ttattttctt cacatcccat ttcttcactc cagtttttgt agctacttca gatttaaacc
      901 actcctcaat catatcaaaa g
//
