LOCUS       sequence-120770-        3327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neisseria meningitidis Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3327)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX405 Neisseria meningitidis Cas9
FEATURES             Location/Qualifiers
     source          1..3327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             76..3318
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="AAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVRVF
                     ERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIKS
                     LPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVAD
                     NAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEFG
                     NPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTK
                     LNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDN
                     AEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLK
                     DRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNTE
                     EKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRKE
                     IEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLGR
                     LNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEFK
                     ARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKRV
                     FASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAFD
                     GKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKLRTLLAEK
                     LSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDL
                     EKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKT
                     GVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQL
                     IDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEG
                     IGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
ORIGIN
        1 accggtgcca ccatgtaccc atacgatgtt ccagattacg cttcgccgaa gaaaaagcgc
       61 aaggtcgaag cgtccgctgc cttcaaacct aaccctatca actacatcct ggggctggac
      121 attggaatcg cttcagtggg ctgggctatg gtggaaatcg acgaggatga aaaccctatt
      181 tgcctgatcg acctgggggt cagagtgttt gagagggctg aagtgccaaa gacaggagat
      241 agtctggcta tggcacggag actggcaagg tcagtgaggc gcctgactcg acggagagca
      301 cacaggctgc tgcgagctag gcgcctgctg aagcgagagg gagtgctgca ggccgctgac
      361 ttcgatgaaa acgggctgat caagagcctg cccaataccc cttggcagct gagagcagcc
      421 gctctggaca ggaagctgac accactggag tggtctgccg tgctgctgca cctgatcaag
      481 catcgcggat acctgagtca gcgaaaaaac gagggcgaaa cagcagataa ggagctggga
      541 gccctgctga aaggcgtggc tgacaatgcc cacgctctgc agaccggcga ttttaggaca
      601 cccgcagaac tggccctgaa caagttcgag aaagaatccg ggcacattcg aaatcagcgg
      661 ggagactatt ctcatacctt cagtcgcaag gatctgcagg ccgagctgat cctgctgttt
      721 gagaagcaga aagagttcgg aaacccccac gtgtctggcg ggctgaaaga gggcatcgaa
      781 acactgctga tgactcagcg gcctgcactg agtggagacg ccgtgcagaa gatgctgggc
      841 cattgtactt ttgagccagc cgaacccaag gcagccaaaa atacctacac agctgagagg
      901 ttcatttggc tgaccaagct gaacaatctg cgcatcctgg agcagggcag cgaacggccc
      961 ctgactgaca ccgagagagc cacactgatg gatgaacctt acaggaagtc taaactgact
     1021 tatgcacagg cacgaaagct gctgggcctg gaggacactg ccttctttaa gggactgcgg
     1081 tacggcaaag ataacgctga ggcaagcacc ctgatggaaa tgaaggctta tcacgcaatc
     1141 tccagagccc tggagaaaga aggcctgaag gacaagaaat cacccctgaa tctgagccct
     1201 gagctgcagg atgaaattgg caccgctttt agcctgttca agactgacga ggatatcacc
     1261 gggcgcctga aagacagaat ccagcccgag atcctggaag ccctgctgaa gcacatctct
     1321 tttgataaat tcgtgcagat tagtctgaag gctctgcgac ggatcgtccc tctgatggag
     1381 cagggcaaaa gatacgacga ggcctgcgct gaaatctacg gggatcatta cggaaagaaa
     1441 aacacagagg aaaaaatcta cctgccccct atccctgccg acgagattcg gaacccagtg
     1501 gtcctgagag ccctgtcaca ggctagaaaa gtgatcaacg gggtggtcag aagatacgga
     1561 agccccgcaa gaatccacat tgagactgcc cgcgaagtgg ggaagtcctt taaagaccgc
     1621 aaggagatcg aaaagcgaca ggaggaaaac agaaaagata gggagaaggc tgcagccaaa
     1681 ttccgggaat actttccaaa tttcgtggga gagcccaagt caaaagacat cctgaagctg
     1741 agactgtacg agcagcagca cggcaagtgt ctgtatagcg ggaaagaaat taacctggga
     1801 aggctgaatg agaagggcta tgtggaaatc gatcatgccc tgccttttag tcgcacatgg
     1861 gacgattcat tcaacaataa ggtcctggtg ctgggcagtg agaaccagaa caaggggaac
     1921 cagactcctt acgaatattt caacggcaag gacaacagcc gggagtggca ggagttcaag
     1981 gcccgggtgg agacctcccg cttcccacga tctaagaaac agcggattct gctgcagaag
     2041 tttgacgagg atgggttcaa agaaagaaac ctgaatgaca ccagatacgt gaatagattt
     2101 ctgtgccagt tcgtcgcaga tcgcatgcga ctgacaggaa aaggcaagaa acgggtgttt
     2161 gccagcaacg gccagatcac taatctgctg agagggttct ggggactgcg gaaggtgaga
     2221 gccgagaacg acaggcacca tgccctggat gctgtggtcg tggcttgtag cactgtggca
     2281 atgcagcaga agattaccag gtttgtccgc tataaagaga tgaatgcctt cgacgggaag
     2341 acaatcgata aagagactgg agaagtgctg caccagaaga cacattttcc tcagccctgg
     2401 gagttctttg cccaggaagt gatgatccgc gtctttggca agcctgacgg gaaaccagag
     2461 ttcgaggaag ctgatacccc agagaagctg cggacactgc tggcagaaaa actgagctcc
     2521 agacccgagg ccgtgcacga atacgtcacc cccctgttcg tgtcaagggc acctaaccgc
     2581 aagatgagcg ggcagggaca catggagacc gtgaaatccg ctaagcggct ggacgaaggc
     2641 gtctctgtgc tgagagtgcc actgacacag ctgaagctga aggacctgga gaagatggtc
     2701 aatcgggaga gagaacccaa gctgtatgag gcactgaaag ccaggctgga agcccacaag
     2761 gacgatccag caaaagcctt tgctgagccc ttctacaaat atgacaaggc tggaaacaga
     2821 actcagcagg tgaaggcagt cagggtggaa caggtccaga aaaccggcgt ctgggtgagg
     2881 aaccataatg ggatcgctga taatgcaaca atggtgcggg tggacgtctt tgagaaaggc
     2941 gataagtact atctggtgcc tatctacagc tggcaggtcg caaagggcat cctgccagac
     3001 cgagctgtcg tgcagggaaa agacgaggaa gattggcagc tgattgacga tagcttcaac
     3061 tttaagttct ccctgcaccc taatgatctg gtggaggtca tcaccaagaa agctagaatg
     3121 tttgggtact tcgcatcctg ccatcgcggc acagggaaca tcaatattcg aatccacgac
     3181 ctggatcata aaattggcaa gaacgggatt ctggagggaa tcggcgtgaa gaccgccctg
     3241 tctttccaga aatatcagat cgacgagctg ggcaaggaaa ttcgaccatg tcggctgaag
     3301 aaacgaccac ccgtgcggta agaattc
//
