LOCUS       sequence-120774-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PRORP2 (At2g16650), full coding sequence + 
            C-term. His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Pavlova LV, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann 
            RK
  TITLE     tRNA processing by protein-only versus RNA-based RNase P: kinetic 
            analysis reveals mechanistic differences.
  JOURNAL   Chembiochem. 2012 Oct 15;13(15):2270-6. doi: 10.1002/cbic.201200434.
            Epub 2012 Sep 13.
  PUBMED    22976545
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201200434"; journalName: "Chembiochem"; date: 
            "2012-10-15- 15"; volume: "13"; issue: "15"; pages: "2270-6"
COMMENT     Sequence Label: pET28-At_PRORP2-His
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atataccatg gctgcttctg atcaacaccg
       61 gtctcgccgt cacgacgaga gctcttcaag acccaacaag aagaagaagg taagcagaaa
      121 cccagaaaca aatctcctct tcaatctcaa ttcatgctcc aagtccaagg acctctcagc
      181 tgcattagca ctttatgatg cagccatcac ttccagtgaa gtacgtctca gccagcaaca
      241 ttttcaaacg cttctgtacc tatgctcagc ttccattact gacatatctc ttcaatacct
      301 tgccattgac cgtggtttcg agatttttga tcgtatggtt agttcgggga taagtcctaa
      361 tgaggcatct gttacttcag tggctagact ggctgctgct aaaggtaatg gtgattatgc
      421 tttcaaggtt gttaaggagt ttgtttctgt tggaggtgtg tcgattcctc gtctgaggac
      481 atatgcgccg gctttgctct gtttctgcga gaagttggag gctgaaaagg gttatgaggt
      541 agaagagcac atggaagctg caggtattgc attggaggag gcagagatat cggctttgtt
      601 gaaggtaagt gctgccacgg gtcgagaaaa caaggtttat agatatttac ataagttgag
      661 ggaatatgtt ggctgcgtta gtgaagaaac tttgaaaatt attgaggaat ggttctgtgg
      721 agaaaaagct ggtgaggttg gtgataatgg aattggttct gatgttggga tgcttagaga
      781 agctgttttg aataatggag gtgggtggca cgggcatggg tgggttgggg agggtaaatg
      841 gactgtaaag aaaggtaatg ttagctcaac aggaagatgt ttgagctgca gtgagcagtt
      901 agcttgtgta gataccaatg aggtagaaac acagaagttt gtggattctt tggtggcttt
      961 ggctatggat aggaagacaa agatgaattc atgtgagaca aatgtggtct tcagcg
//
