LOCUS       sequence-120779-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PRORP3 (At4g21900), full coding sequence + 
            C-term. His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Pavlova LV, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann 
            RK
  TITLE     tRNA processing by protein-only versus RNA-based RNase P: kinetic 
            analysis reveals mechanistic differences.
  JOURNAL   Chembiochem. 2012 Oct 15;13(15):2270-6. doi: 10.1002/cbic.201200434.
            Epub 2012 Sep 13.
  PUBMED    22976545
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201200434"; journalName: "Chembiochem"; date: 
            "2012-10-15- 15"; volume: "13"; issue: "15"; pages: "2270-6"
COMMENT     Sequence Label: pET28-At_PRORP3-His
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catggctggt actgataacc
       61 gccgctctcg ccacgacgac gaaagcccta aaaatcccaa caagaagaag aaaggaaacc
      121 gtaatccaga aaagagtctc cttatcaatc ttcattcatg ttccaagaga aaggatctct
      181 cagccgcatt ggctctttat gatgcagcta tcacttccag tgacattcgc ctcaaccagc
      241 aacacttcca atcccttctt tacctctgct ccgcgttcat tagtgaccca tctcttcaaa
      301 ccgttgccat tgaccgtggc tttcaaattt ttgaccgtat ggttagttct gggataagtc
      361 ctaatgaatc atctgtcact gcagttgcac gactagctgc tgccaagggt gatggagact
      421 atgctttcaa gcttgttaaa gaccttgttg ctgttggtgg tgtatcggtc cctcgtctga
      481 gaacttatgc tccggctttg ctctgtttct gtgatacttt ggaggctgaa aagggttatg
      541 aagtggaaga tcacatggat gcttcaggta ttgtgttgga ggaggcagag atctcggctt
      601 tgctaaaggt aagcgctgcc acaggccgag aaaacaaggt ttatagatat ctgcagaagt
      661 taagagaatg tgttggctgc gttagtgaag aaacttcaaa agcaatcgag gaatggtttt
      721 atggagtgaa agctagcgaa gttagtgata atgggattgg ttctgatatt gagctgctta
      781 gagcagctgt tttgaaaaat gggggtgggt ggcatggtct tgggtgggtt ggagaaggca
      841 aatggattgt gaagaaaggt aatgttagct cagccgggaa atgtttgagc tgtgatgagc
      901 atttggcttg tgttgatacc aatgaggtag agactgaaga ttttgtgaat tctttggtga
      961 ctttggctat ggagaggaag gcaaagatga attcatgcga gc
//
