LOCUS       sequence-120784-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Venus-LUMP fusion with thrombin cleavage 
            site, a FRET-based thrombin sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 274_pSKB3-Venus-LUMP
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(614..631)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(680..701)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcctac cattcattta
       61 aaatttcaac gttcactttc acatcttttg ctaacgatgc aatgttagta ctcgctaaca
      121 aatcacgcga gtaattcaat gtaataatgc tgtctgacat atcagtgata ggcaaagaga
      181 tcccatcaat tccaatatca tctttcactg ttaagttttc agctaaccct ttagggatat
      241 caatcagtac agaaaaacct gcgtcattct cttctattgc tgcgaccagc gcagtgcctt
      301 taatatttcc tgttaatcca ccacggccaa ccacttcacc aaatttaggg tggatctcta
      361 ggtttacctg atcgccttct tttaatccat caaatgttgt cgtacccaat gcttggtcga
      421 tatcaaagta caccatatcg cctaaaatac ggacaactgt caatgaacaa ccatttacta
      481 acattacagt atctacatca acaagttgaa acataccctc agggaatgca atcccatgtc
      541 gctgactatc ttcacttttt gaaatggatc gaataacacc acgaccttga acaatacctc
      601 taaagcttcc acgggatcca cgcggaacca ggctagcctt gtatagctcg tccatgccga
      661 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      721 ggtctttgct cagggcggac tggtagctca ggtagtggtt gtcgggcagc agcacggg
//
