LOCUS       sequence-120790-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLCRISPR/Cas9Pubi-H
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    14..188
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt
       61 attgatagaa gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac
      121 atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca cacattatta
      181 tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct atttctttgc
      241 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
      301 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
      361 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
      421 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga
      481 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
      541 ccacggcctc cagaagaaga tgttggcgac ctc
//
