LOCUS       sequence-120796-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Cas9 in plants, cauliflower mosaic virus 35S promoter 
            (P35S), hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLCRISPR/Cas9P35S-H
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    33..207
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
       61 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
      121 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
      181 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat cccggtcggc
      241 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
      301 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
      361 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
      421 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
      481 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
      541 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
      601 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
      661 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
      721 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
      781 cagtt
//
