LOCUS       sequence-120802-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of an optimized Cas9 for genome-editing in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Jao LE, Wente SR, Chen W
  TITLE     Efficient multiplex biallelic zebrafish genome editing using a 
            CRISPR nuclease system.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 5.
  PUBMED    23918387
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 5."
COMMENT     Sequence Label: pCS2-nCas9n
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..59)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             154..162
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgtaatacga ctcactatag ttctagattc cgcggtcaca cctttctctt cttcttagga
       61 gatctcaccg gtgagtcgcc acccagctga gacaggtcaa tccttgtttc gtacaggccg
      121 gtaatactct gatggatcag tgtagcgtcc agcacctcct tagtggaggt gtacctcttt
      181 ctatcgattg tagtgtcgaa gtacttgaaa gctgcaggtg cgcccaggtt tgtcagagtg
      241 aacagatgaa tgatattttc ggcctgctcc ctgatgggct tatctctgtg tttgttgtaa
      301 gcagacagga ccttatccag atttgcgtcg gccaggatca ctcttttaga gaactcgcta
      361 atctgttcaa tgatctcgtc cagataatgc ttgtgctgct ccacaaacag ctgtttctgt
      421 tcgttatcct ctggagatcc tttcagcttc tcgtagtggc ttgccagata caggaagttc
      481 acgtacttag aaggcagggc cagttcattg cctttctgca gctctccagc gcttgccagc
      541 atgcgtttcc tgccgttttc cagctcaaac agactgtact tgggcagttt aatgatcagg
      601 tctttcttca cttccttata acctttggcc tccagaaaat cgattgggtt cttctcgaaa
      661 gagcttctct ccataattgt gatacccagc agctccttga cagatttcag tttcttgctt
      721 ttgcccttct ccaccttagc gaccaccagg acggagtatg ccacagtggg agagtcaaag
      781 cctccatact tcttagggtc ccagtctttc tttctagcga tcagcttatc agagttgcgt
      841 tttggcagaa tactttcttt tgagaaaccg cctgtctgca cctcagtttt cttgacgata
      901 ttcacttgag gcatgctcag gaccttcctc acggttgcaa aatctctgcc tttgtcccac
      961 acgatttcgc ctgtctctcc gtttgtctca atcaggggcc ttttgcggat ttctccattg
     1021 gccagggtga tctctgtctt aaagaaattc atgatgtttg agtaaaagaa atacttggca
     1081 gtagcttttc caatttcctg ctcggacttg gcgatcattt ttctgacatc gta
//
