LOCUS       sequence-120804-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of an optimized Cas9 for genome-editing in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Jao LE, Wente SR, Chen W
  TITLE     Efficient multiplex biallelic zebrafish genome editing using a 
            CRISPR nuclease system.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 5.
  PUBMED    23918387
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 5."
COMMENT     Sequence Label: pCS2-nCas9n
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..74
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     56..73
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcaatgctc gtttagggaa ccgccattct gcctggggac gtcggagcaa gcttgattta
       61 ggtgacacta tagaatacaa gctacttgtt ctttttgcag gatccgatcc aatgtcgacg
      121 ccaccatggc ttctccacct aagaagaaga gaaaggtggg aagcatggat aagaagtata
      181 gcatcggcct ggatattgga actaactccg tgggttgggc agtgattaca gacgactaca
      241 aggtccctag caagaaattt aaggtgctgg gtaacaccga caggcacagc atcaagaaaa
      301 atctgattgg agccctgctg ttcggttctg gagagactgc cgaagcaaca cgcctgaaaa
      361 gaacagcaag aaggcgctat accagaagga agaatagaat ctgttacctg caggagattt
      421 tctctaacga aatggctaag gtggacgatt cattctttca taggctggag gaaagtttcc
      481 tggtcgagga agataagaaa cacgagcgcc atcctatctt tggaaacatt gtggacgagg
      541 tcgcctatca cgaaaaatac ccaaccatct atcatctgcg caagaaactg gctgactcta
      601 ctgataaagc cgacctgaga ctgatctatc tggctctggc ccacatgatt aagttcaggg
      661 gtcattttct gatcgagggc gatctgaacc ccgacaattc cgatgtggac aagctgttca
      721 tccagctggt ccagatttac aatcagctgt ttgaggaaaa ccctattaat gcttccagag
      781 tggacgcaaa agctatcctg tcagccaggc tgtccaagtc acgcagactg gagaacctga
      841 ttgcacagct gcccggagaa aagaggaacg gtctgtttgg aaatctgatc gctctgagtc
      901 tgggcctgac tcctaacttc aaaagcaatt ttgatctggc tgaggacgcc aaactgcagc
      961 tgtcaaagga cacatatgac gatgacctgg ataacctgct ggcacagatc ggagatcagt
     1021 acgctgacct gttcctggct gccaaaaatc tgtccgacgc aatcctgctg tcagatattc
     1081 tgagagtgaa cagcgagatt acaaaagctc ctctgagtgc cc
//
