LOCUS       sequence-120814-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to 
            Ser) with N-term Histag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney 
            BJ, Brash AR
  TITLE     On the relationships of substrate orientation, hydrogen abstraction,
            and product stereochemistry in single and double dioxygenations by 
            soybean lipoxygenase-1 and its Ala542Gly mutant.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38756-66. Epub 2005 Sep 12.
  PUBMED    16157595
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38756-66. 
            Epub 2005 Sep 12"
COMMENT     Sequence Label: pET30Xa-SBL1(160E,NoCys)
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..80
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             87..131
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             162..173
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtctggtgcc acgcggttct ggtatgaaag aaaccgctgc tgctaaattc gaacgccagc
      121 acatggacag cccagatctg ggtaccggtg gtggctccgg tattgagggt cgcatgtttt
      181 cagcaggcca taagatcaaa gggacagtgg tgttgatgcc caagaatgag ttggaagtta
      241 accctgatgg ctcagcagtt gacaacctta atgctttctt gggccgtagt gtctcccttc
      301 agctcattag tgctaccaag gctgatgcac atggaaaagg aaaagttgga aaggatacgt
      361 tcttggaagg gattaatact tcgttaccaa ctttgggagc aggagagtct gcattcaata
      421 ttcattttga atgggacgga agcatgggaa tccccggtgc gttttacata aagaactaca
      481 tgcaagttga gttcttcctc aagagtttga ctcttgaagc catttcaaac caaggaacca
      541 tccgctttgt ttccaactca tgggtttata acactaaact ttacaaaagc gtgcgcattt
      601 tctttgccaa ccatacatat gtacctagtg agacaccagc accacttgtg gagtacagag
      661 aagaagaatt gaagagttta agaggaaatg gaacgggaga gcgcaaggaa tatgatagga
      721 tctatgatta tgatgtctac aatgatt
//
