LOCUS       sequence-120821-        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: CMV-Brainbow-2.1 R
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             114..830
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     CDS             882..902
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(942..962)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(942..960)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(980..997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    986..1097
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatcgagata
       61 acttcgtata gcatacatta tacgaagtta taccggatag ggagacccgc accatggtga
      121 gcaagcagat cctgaagaac accggcctgc aggagatcat gagcttcaag gtgaacctgg
      181 agggcgtggt gaacaaccac gtgttcacca tggagggctg cggcaagggc aacatcctgt
      241 tcggcaacca gctggtgcag atccgcgtga ccaagggcgc ccccctgccc ttcgccttcg
      301 acatcctgag ccccgccttc cagtacggca accgcacctt caccaagtac cccgaggaca
      361 tcagcgactt cttcatccag agcttccccg ccggcttcgt gtacgagcgc accctgcgct
      421 acgaggacgg cggcctggtg gagatccgca gcgacatcaa cctgatcgag gggatgttcg
      481 tgtaccgcgt ggagtacaag ggccgcaact tccccaacga cggccccgtg atgaagaaga
      541 ccatcaccgg cctgcagccc agcttcgagg tggtgtacat gaacgacggc gtgctggtgg
      601 gccaggtgat cctggtgtac cgcctgaaca gcggcaagtt ctacagctgc cacatgcgca
      661 ccctgatgaa gagcaagggc gtggtgaagg acttccccga gtaccacttc atccagcacc
      721 gcctggagaa gacctacgtg gaggacggcg gcttcgtaga gcagcacgag accgccatcg
      781 cccagctgac cagcctgggc aagcccctgg gcagcctgca cgagtgggtg gagctctccg
      841 gactcagatc tcgaccgcgg gcccgcgacc ctcccgtggg ccccaagaag aagcgcaagg
      901 tgggcgccta agatccaccg gatctagata atagggtacc acctttagtg agggttaatt
      961 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     1021 ccctcccccg tgccttcctt gaccctggaa ggtgccactc tcactgtcct ttcctaataa
     1081 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     1141 gggcagg
//
