LOCUS       sequence-120822-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-LIN-29-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg agaggatcgc atcaccatca ccatcacggg ccctctagaa
      121 tggatcaaac tgtactagat tcggcattca actctccagt ggactctgga atagctggaa
      181 ccaccactgg ctccggatct accacccatt ttggtgttgg aacaaacttg agatcatcaa
      241 gtcggtcaac cgacggcact gactcaacgg acggtgccaa ctcggacaat gtgaccggat
      301 caactggatc cacaccagca catcattcga tcactaattt gaatatggct ttgtcgcaac
      361 actccattga ctcggcaact gctgcttcat cgacaaaccc gtttccgcat tttaatcaag
      421 cggatctact gaacttccat cagaattctc tcctgccaca tcatatgttt tcacaatttg
      481 gaagatatcc acagttcgaa caaaagccgg acgtgggggt gcttcagcaa cagatgcaga
      541 tgcgggaagc aaaaccttac aagtgcacgc agtgtgtcaa ggcatttgcc aactcatcct
      601 atctctccca acacatgaga attcatcttg gcatcaagcc atttgggcca tgtaactatt
      661 gcggcaagaa gttcactcag ctatcacacc ttcagcagca cattcgaaca catacaggag
      721 agaaaccgta taagtgcaaa ttcacgggat gcgacaaagc attctcccag ttaagcaatc
      781 tccaatctca ctctcgatgc catcaaactg ataagccatt caaatgtaac agttgctata
      841 aatgcttcac cgacgagcag agcctcttgg atcacattcc gaagcacaaa gagtcaaagc
      901 atctgaagat tcacatttgc ccgttt
//
