LOCUS       sequence-120823-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 WAVE 2
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..43)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(186..205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(187..205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        237..341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             526..904
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(560..579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tctcgccctt gcttgtgccg tggtgatggt ggtgatggtg gtgaccagag gtgcccatgg
       61 tttcacttac gtgtttaatg tctttacaga ctttatacat tactgtcatg gttaaataaa
      121 ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaacttaa
      181 gatgtcccta tagtgagtcg tattagacgt caggtggcac ttttcgggga aatgtgcgcg
      241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      961 actggatgga
//
