LOCUS       sequence-120824-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 WAVE 2
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagctgg gttatcggac cagtcgtcct catcaaattc agaggagtca tcttctgagt
      241 cactgtactc aacagcaatg cgacgagaca agatggtggc cacgtcattg cccacaacat
      301 cccgcttctc ttgttcccgc tgctcctcaa ccctgcgcag ctgaaaacct tgacggatgg
      361 ctgaaagcag gtcgctacgg gcatcgctca cggcaggcaa ggaggacttg ggcttggtgg
      421 tatcagaaag cggtggtggt atagcaggct ggccatctgc accagtgaaa gggggaggag
      481 ggggccccgg aggaggagga ggagggggag gaggaggtgc tcctcctgtt ggctgggaca
      541 agggaggtgg tggagcaaac ccgggtttag ggcctggtgg agagcctaga ggaggagctg
      601 gtggtggatg gcttgggctg
//
