LOCUS       sequence-120828-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PPARG
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgctc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggtagtacaa gtccttgtag atctcctgca ggagcgggtg aagactcatg
      241 tctgtctccg tcttcttgat cacctgcagt agctgcacgt gttccgtgac aatctgtctg
      301 aggtctgtca ttttctggag cagcttggca aacagctgtg aggactcagg gtggttcagc
      361 ttcagctgga gctccagggc ttgtagcagg ttgtcttgaa tgtcttcaat gggcttcaca
      421 ttcagcaaac ctgggcggtc tccactgaga ataatgacag caataaatat tgccaagtcg
      481 ctgtcatcta attccagtgc attgaacttc acagcaaact caaacttggg ctccataaag
      541 tcaccaaaag gctttcgcag gctctttaga aactcccttg tcatgaagcc ttggccctcg
      601 gatatgagaa ccccatcttt attcatcaag gaggccagca ttgtgtaaat gatctcgtgg
      661 actccatatt tgaggagagt tacttggtcg ttcaagtcaa gatttacaaa accaggaatg
      721 cttttggcat actctgtgat ctcctgcaca gcctccacgg agcgaaactg gcagccctga
      781 aagatgcgga tggccacctc tttgctctgc tcctgcaggg gggtgatgtg ttt
//
