LOCUS       sequence-120831-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CTTN
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagctgg gttctgccgc agctccacat agttggctgg gaagagcccg taccggccct
      241 tgcacacccc gcgccaccag ccgtcgtcaa tcatctcgat gttggtgatg atgtcatcag
      301 ggtcaaatga gatctcatca tcgcccgcag cctggtagtc gtacagggcg acggctgtga
      361 tccccagatc gttctcgtac tcatcgtagg tgctgtcctc tgcgggatag tggcccgggg
      421 cctctgcgct ttcatagaca gcctctgtgg cataggccag gccctgctgg ctgctggcct
      481 ctcggtagtc agcggcctcc atgctgtaca cgggctccgg ctccgtccca ctcacagggc
      541 ctctgtagct cagctctgcc ttgaaggaag ccgcatcctc atagacgggg ctcgagggca
      601 gcctctcctc ggttggctga ggtgcgggcg acacaggggg cgtttgcgtt ttggctctgg
      661 cttgctcctc cagcttcctc ctggcctctt cctgctcctg ccgctccttg gccatccgct
      721 gggctctctc cgcctccgcc ttccgcctgt cctcctgctc tttctcctta gcgaggtttt
      781 caaagttagc tctgatgtta cttgttttgc tggtcacagc ttcgacaggt actgtcttct
      841 ggtaggcaga ggacacctgg gtgacatcct caaaggttga cgcattctta tccatccgat
      901 ccttctgcac cccatacttc ccgccgaatc ctttggagt
//
