LOCUS       sequence-120835-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 HDAC1
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcattctat agagaagtac acgtaaaagt aagactcgtc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggcaggccaa cttgacctcc tccttgaccc ctttggcttc tggcttctcc
      241 tccttggttt tctcctcttc ggtgacttct ttcttctcct ctgggtcttt ctctttttca
      301 tcctctgttt tgactctctt ggcttttttg aagttggaag agttcttgcg gcccccctct
      361 ccctcctctt cagaatcgga gaactcttcc tcacaggcaa ttcgtttgtc agaggagcag
      421 atcgagatgc gcttgtcagg gtcgtcttcg tcctcatcgc cactctcctc agggatggcg
      481 tcctcaggaa tcgcctgcat ttggacccca ggtgcgtgcg gcagcattct aaggttctca
      541 aacagtcgct gtttgatctt ctccaggtac tcattcgtgt tctggttagt catattggaa
      601 ggactgatgt ggagcttgaa atctggtcca aagtattcaa agtagtcatt gtatggaagc
      661 tcattaggga tctccgtatc cagggccaca gctgtctcat atgtccagca ccgggcaacg
      721 ttacgaatgg tgtaaccacc gcctcccagc atcagcatag gcaggttaaa gctcttgaca
      781 aattccacac acttggcgtg tcctttgata gttagattga agcaacctaa ccgatcccca
      841 gaatagggag tctgagccac actgtaagac caccgcacta ggctggaaca tctccattac
      901 tttggacatg accggcttga aaatggcctc ataggactcg tcatcaatcc
//
