LOCUS       sequence-120837-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with H134R mutation 
            fused to mCherry driven by CaMKIIa promoter for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Apr 5. 446(7136):633-9."
COMMENT     Sequence Label: pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      411..420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agggttgctc aggcggataa ggatgacagg acaagtgagc agccactctg catagcgcag
       61 ccactgcacc cggtgtcctg tggcaaggta gagcatagag ggattcttaa actcaaaaaa
      121 gaactcgaga atcaccttaa ccatttcaat ggcgcacaca tagatctcct cccagccgca
      181 tgtagatttc caggtttggt aggcatagaa catcagcagc aaaatgctga atcctgctgc
      241 aagccactgc aggacatttg acgcggtctg agcgccgttc gtgccgcgag attcaatcca
      301 tccggcacag taacattgat cctcagggac caggacggac ccgttcacca ccacaggatt
      361 agtaacgaac aaaagttcgc gtccgacggc agacaaagcg ccgccatagt ccatggtggc
      421 ctaccggtac ccggggatcc cccgctgccc ccagaactag gggccactcg cctgcccgtg
      481 ctcctgagtg caaacggaga accggcttga ctgacgagct tggggcttct gagcagggca
      541 ctgtggctgc tcacagcctc gcgagccttc gtcagcatcc aggtccccat ggcaaccacc
      601 tccgaaacgc cctgttcccg ttgccccggt aactgcctcc ccaacacctg cctgcctttc
      661 actttaaaac ctgctcctct ttgccctggc cttatataca caatactgca gg
//
