LOCUS       sequence-120838-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression construct for targeting the murine ROSA26 locus. It
            recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK
            mCherryROSAbsr plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     rosa26 knock-in plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS-ROSAf
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             129..152
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             153..179
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattccgcc accatggctt cctcccctcc aaagaaaaag agaaaggttg
      121 cggccgctga ctacaaggat gacgacgata aaagttggaa ggacgcaagt ggttggtcta
      181 gaatgcatgc ggccccgcga cggcgtgctg cgcaaccctc cgacgcttcg ccggccgcgc
      241 aggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc aaaccgaagg
      301 tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt acacacgcgc
      361 acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc acgtatcagc
      421 acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc ggcaaacagt
      481 ggtccggcgc acgcgccctg gaggccttgc tcacggatgc gggggagttg agaggtccgc
      541 cgttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc gtgaccgcaa
      601 tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctgaac ctgaccccgg
      661 accaagtggt ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc
      721 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
      781 ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
      841 gcca
//
